Thermophilic bacteria as a source of polyhydroxyalkanoates
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU142642" target="_blank" >RIV/00216305:26310/21:PU142642 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Thermophilic bacteria as a source of polyhydroxyalkanoates
Popis výsledku v původním jazyce
Thermophilic bacteria are microorganisms capable of growing in extreme conditions, they survive temperatures above 45 °C and can be found in various habitats. These organisms have been studied because of their unique abilities to survive in harsh conditions and they also stand as promising biotechnological producers of various metabolites from thermostable enzymes to biopolymers such as polyhydroxyalkanoates (PHAs). One of the advantages of using extremophilic organisms in biotechnological processes is the prevention of possible contamination by mesophilic microorganisms, which results in a reduced requirement for sterilization. In our work, we focused on the morphological study of thermophilic bacteria Schlegelella thermodepolymerans and Thermomonas hydrothermalis using cryo-SEM and also on the evaluation of the amount of PHAs in microbial cells using gas chromatography. For cryo-SEM analysis, bacterial cells were fixed using high pressure freezing method without using any cryo-protectants. Frozen sa
Název v anglickém jazyce
Thermophilic bacteria as a source of polyhydroxyalkanoates
Popis výsledku anglicky
Thermophilic bacteria are microorganisms capable of growing in extreme conditions, they survive temperatures above 45 °C and can be found in various habitats. These organisms have been studied because of their unique abilities to survive in harsh conditions and they also stand as promising biotechnological producers of various metabolites from thermostable enzymes to biopolymers such as polyhydroxyalkanoates (PHAs). One of the advantages of using extremophilic organisms in biotechnological processes is the prevention of possible contamination by mesophilic microorganisms, which results in a reduced requirement for sterilization. In our work, we focused on the morphological study of thermophilic bacteria Schlegelella thermodepolymerans and Thermomonas hydrothermalis using cryo-SEM and also on the evaluation of the amount of PHAs in microbial cells using gas chromatography. For cryo-SEM analysis, bacterial cells were fixed using high pressure freezing method without using any cryo-protectants. Frozen sa
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů