Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F22%3APU145818" target="_blank" >RIV/00216305:26310/22:PU145818 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2076-2607/10/6/1179/htm" target="_blank" >https://www.mdpi.com/2076-2607/10/6/1179/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms10061179" target="_blank" >10.3390/microorganisms10061179</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

Popis výsledku v původním jazyce

Cellular autofluorescence is usually considered to be a negative phenomenon because it can affect the sensitivity of fluorescence microscopic or flow cytometric assays by interfering with the signal of various fluorescent probes. Nevertheless, in our work, we adopted a different approach, and green autofluorescence induced by flavins was used as a tool to monitor fermentation employing the bacterium Cupriavidus necator. The autofluorescence was used to distinguish microbial cells from abiotic particles in flow cytometry assays, and it was also used for the determination of viability or metabolic characteristics of the microbial cells. The analyses using two complementary techniques, namely fluorescence microscopy and flow cytometry, are simple and do not require labor sample preparation. Flavins and their autofluorescence can also be used in a combination with other fluorophores when the need for multi-parametrical analyses arises, but it is wise to use dyes that do not emit a green light in order to not interfere with flavins’ emission band (500–550 nm).

Název v anglickém jazyce

Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

Popis výsledku anglicky

Cellular autofluorescence is usually considered to be a negative phenomenon because it can affect the sensitivity of fluorescence microscopic or flow cytometric assays by interfering with the signal of various fluorescent probes. Nevertheless, in our work, we adopted a different approach, and green autofluorescence induced by flavins was used as a tool to monitor fermentation employing the bacterium Cupriavidus necator. The autofluorescence was used to distinguish microbial cells from abiotic particles in flow cytometry assays, and it was also used for the determination of viability or metabolic characteristics of the microbial cells. The analyses using two complementary techniques, namely fluorescence microscopy and flow cytometry, are simple and do not require labor sample preparation. Flavins and their autofluorescence can also be used in a combination with other fluorophores when the need for multi-parametrical analyses arises, but it is wise to use dyes that do not emit a green light in order to not interfere with flavins’ emission band (500–550 nm).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA19-20697S" target="_blank" >GA19-20697S: Řízená evoluce bakterií produkujících polyhydroxyalkanoáty</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

21

Strana od-do

1-21

Kód UT WoS článku

000930526300001

EID výsledku v databázi Scopus

2-s2.0-85131573880