Utilization of wine lees as a substrate for polyhydroxyalkanoates production by employing halophilic and thermophilic bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F26%3A0199338" target="_blank" >RIV/00216305:26310/26:0199338 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0141391025003532" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141391025003532</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.polymdegradstab.2025.111524" target="_blank" >10.1016/j.polymdegradstab.2025.111524</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of wine lees as a substrate for polyhydroxyalkanoates production by employing halophilic and thermophilic bacteria

Popis výsledku v původním jazyce

The increasing demand for sustainable alternatives to petroleum-based plastics has intensified research into polyhydroxyalkanoates (PHAs), biodegradable polyesters produced by microorganisms. Large-scale production remains economically challenging due to the high cost of substrates and the requirement for sterile fermentation conditions. The concept of Next-Generation Industrial Biotechnology suggests employing extremophilic microorganisms that can thrive in harsh conditions and reduce operational costs by minimizing contamination risks. This study explores the use of distilled wine lees, a nutrient-rich by-product of Prosecco winemaking, as a sustainable carbon source for PHAs production by extremophilic microorganisms. Among the 17 thermophilic and 9 halophilic screened strains, Caldimonas thermodepolymerans DSM 15344 exhibited the highest growth and PHAs accumulation on wine lees. Under optimized conditions, this strain achieved a cell dry weight (CDW) of 2.42 g/L and produced 1.17 g/L of poly(3-hydroxybutyrate) (PHB) homopolymer, representing 49.30 % of the CDW. Polymer characterization confirmed physicochemical and thermal properties were comparable to those obtained from standard sugar-based media, such as xylose. In simulated repeated-batch cultivation on DWL, C. thermodepolymerans DSM 15344 maintained stable performance over four subsequent cycles. These results underscore the suitability of C. thermodepolymerans DSM 15344 for cost-effective, sustainable PHAs production from agro-industrial waste and affirm the biotechnological relevance of thermophilic extremophiles in circular bioeconomy strategies.

Název v anglickém jazyce

Utilization of wine lees as a substrate for polyhydroxyalkanoates production by employing halophilic and thermophilic bacteria

Popis výsledku anglicky

The increasing demand for sustainable alternatives to petroleum-based plastics has intensified research into polyhydroxyalkanoates (PHAs), biodegradable polyesters produced by microorganisms. Large-scale production remains economically challenging due to the high cost of substrates and the requirement for sterile fermentation conditions. The concept of Next-Generation Industrial Biotechnology suggests employing extremophilic microorganisms that can thrive in harsh conditions and reduce operational costs by minimizing contamination risks. This study explores the use of distilled wine lees, a nutrient-rich by-product of Prosecco winemaking, as a sustainable carbon source for PHAs production by extremophilic microorganisms. Among the 17 thermophilic and 9 halophilic screened strains, Caldimonas thermodepolymerans DSM 15344 exhibited the highest growth and PHAs accumulation on wine lees. Under optimized conditions, this strain achieved a cell dry weight (CDW) of 2.42 g/L and produced 1.17 g/L of poly(3-hydroxybutyrate) (PHB) homopolymer, representing 49.30 % of the CDW. Polymer characterization confirmed physicochemical and thermal properties were comparable to those obtained from standard sugar-based media, such as xylose. In simulated repeated-batch cultivation on DWL, C. thermodepolymerans DSM 15344 maintained stable performance over four subsequent cycles. These results underscore the suitability of C. thermodepolymerans DSM 15344 for cost-effective, sustainable PHAs production from agro-industrial waste and affirm the biotechnological relevance of thermophilic extremophiles in circular bioeconomy strategies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GA22-10845S" target="_blank" >GA22-10845S: Studium role polyhydroxyalkanoátů u bakterie Schlegelella thermodepolymerans – slibného bakteriálního kandidáta pro biotechnologie nové generace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

POLYMER DEGRADATION AND STABILITY

ISSN

0141-3910

e-ISSN

1873-2321

Svazek periodika

—

Číslo periodika v rámci svazku

241

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

001528423600002

EID výsledku v databázi Scopus

—