Revealing the role of zinc(II) in prostate cancer pathogenesis
Identifikátory výsledku
Kód výsledku v IS VaVaI
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Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Revealing the role of zinc(II) in prostate cancer pathogenesis
Popis výsledku v původním jazyce
Current diagnostic procedures do not enable us to distinguish between clinically latent forms of prostate cancer from aggressive forms, the most common cancer in males. The key to understand the different behavior of these forms is in its molecular mechanisms. Current literature highlights the role of zinc(II) ions in the development and pathogenesis of this cancer. Our objective was to identify diverse metabolical pathways of these forms of cancer and determine prognostic markers of high-grade form of prostate cancer. We determined genes alpha-methylacyl-CoA racemase (AMACR), caveolin-1 (Cav-1), metallothionein (MT), p53, NF-κB, c-FOS, c-JUN, Ki-67, ZIP1 and ZnT-1 on RNA and protein level in the sera of 133 men (82 tumorous and 51 healthy) and in cell lines derived from aggressive form of cancer. The level of these genes was determined and compared to prostatic specific antigen, widely used prostate cancer biomarker. We identified significantly (p < 0.05) decreased (> 4-fold) RNA level of Cav-1 NF-κB, c-FOS a c-JUN and significantly elevated (> 2-fold) RNA level of MT, AMACR, PSA, Ki-67, MMP-9 and ZIP1 and ZnT-1 (> 25-fold) in tumorous cell line 22Rv1 in comparison to healthy cell line PNT1A. On the protein level, we identified significant (> 20-fold) decrease of MT in cell lines. In contrary, serum MT level was significantly 4-fold increased. Furthermore, we identified insignificant changes in serum Cav-1 and AMACR. However, Cav-1 level was significantly increased in high stage TNM T4 patients and positively correlated with grading (r = 0.29 at p = 0.028). Thus, we may consider the combination of Cav-1 and MT as a high risk prostate cancer biomarker.
Název v anglickém jazyce
Revealing the role of zinc(II) in prostate cancer pathogenesis
Popis výsledku anglicky
Current diagnostic procedures do not enable us to distinguish between clinically latent forms of prostate cancer from aggressive forms, the most common cancer in males. The key to understand the different behavior of these forms is in its molecular mechanisms. Current literature highlights the role of zinc(II) ions in the development and pathogenesis of this cancer. Our objective was to identify diverse metabolical pathways of these forms of cancer and determine prognostic markers of high-grade form of prostate cancer. We determined genes alpha-methylacyl-CoA racemase (AMACR), caveolin-1 (Cav-1), metallothionein (MT), p53, NF-κB, c-FOS, c-JUN, Ki-67, ZIP1 and ZnT-1 on RNA and protein level in the sera of 133 men (82 tumorous and 51 healthy) and in cell lines derived from aggressive form of cancer. The level of these genes was determined and compared to prostatic specific antigen, widely used prostate cancer biomarker. We identified significantly (p < 0.05) decreased (> 4-fold) RNA level of Cav-1 NF-κB, c-FOS a c-JUN and significantly elevated (> 2-fold) RNA level of MT, AMACR, PSA, Ki-67, MMP-9 and ZIP1 and ZnT-1 (> 25-fold) in tumorous cell line 22Rv1 in comparison to healthy cell line PNT1A. On the protein level, we identified significant (> 20-fold) decrease of MT in cell lines. In contrary, serum MT level was significantly 4-fold increased. Furthermore, we identified insignificant changes in serum Cav-1 and AMACR. However, Cav-1 level was significantly increased in high stage TNM T4 patients and positively correlated with grading (r = 0.29 at p = 0.028). Thus, we may consider the combination of Cav-1 and MT as a high risk prostate cancer biomarker.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MENDELNET 2012
ISBN
978-80-7375-836-3
ISSN
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e-ISSN
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Počet stran výsledku
8
Strana od-do
934-941
Název nakladatele
Neuveden
Místo vydání
Neuveden
Místo konání akce
Mendel Univ, Fac Agron, Brno
Datum konání akce
21. 11. 2012
Typ akce podle státní příslušnosti
CST - Celostátní akce
Kód UT WoS článku
000366461200107