ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES

Popis výsledku v původním jazyce

The objective of the experiment was to establish and optimize an electrochemical method of low molecular weight antioxidants assay using square wave voltammetry performed on screen printed sensors with carbon working electrodes. The potential range of square wave voltammetry was 0 - 1.1 V. The optimal values for the assay, potential step 5 mV, amplitude 5 mV, frequency 1 Hz and volume of sample 20 µl, were found. The linearity and sensitivity of the method were verified by standard antioxidants - ascorbic acid, trolox, N-acetylcysteine and melatonin. The suitability for low molecular weight antioxidants` assay in human serum samples was confirmed by standard ferric reducing antioxidant power test (FRAP). Limits of detection were 0.3 mmol/l for ascorbic acid, 0.04 mmol/l for melatonin, 0.03 mmol/l for trolox, and 0.07 mmol/l for N-acetylcysteine. There was apparent a strong correlation between square wave voltammetry and the FRAP value as the correlation coefficient was 0.9778.

Název v anglickém jazyce

ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES

Popis výsledku anglicky

The objective of the experiment was to establish and optimize an electrochemical method of low molecular weight antioxidants assay using square wave voltammetry performed on screen printed sensors with carbon working electrodes. The potential range of square wave voltammetry was 0 - 1.1 V. The optimal values for the assay, potential step 5 mV, amplitude 5 mV, frequency 1 Hz and volume of sample 20 µl, were found. The linearity and sensitivity of the method were verified by standard antioxidants - ascorbic acid, trolox, N-acetylcysteine and melatonin. The suitability for low molecular weight antioxidants` assay in human serum samples was confirmed by standard ferric reducing antioxidant power test (FRAP). Limits of detection were 0.3 mmol/l for ascorbic acid, 0.04 mmol/l for melatonin, 0.03 mmol/l for trolox, and 0.07 mmol/l for N-acetylcysteine. There was apparent a strong correlation between square wave voltammetry and the FRAP value as the correlation coefficient was 0.9778.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

20601 - Medical engineering

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MENDELNET 2012

ISBN

978-80-7375-836-3

ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

1225-1232

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Mendel Univ, Fac Agron, Brno

Datum konání akce

21. 11. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

000366461200143