Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU104844" target="_blank" >RIV/00216305:26620/13:PU104844 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/13:00204831

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector

Popis výsledku v původním jazyce

Detection of the specific sequences of pandemic-causing viral nucleic acids is area attracting numerous scientists worldwide. In this study, we focused on the isolation and detection of oligonucleotide specific sequences of life-threatening viruses (HIV(GAGCAGTGGGAATA), Influenza (TAATAACCATTGGA) and Ebola (ACCTCACTAGAAAA)) by flow injection analysis with electrochemical detection coupled with paramagnetic particles based isolation. All of used viral genomic sequences contained A6 adenine repetition onthe 5 end. This modification enables us to bind them on paramagnetic particle anchoring T25 (MPs-(dT)25). The isolated sequences were subsequently hydrolysed by optimized method using microwaves to obtain single adenines. The obtained samples were detected by microfluidic tool based on stopped flow injection analysis with glassy carbon electrode in the presence of Cu(II)/or CuS nanoparticles to enhance sensitivity and to lower detection limit for adenine. Basic detection parameters as a

Název v anglickém jazyce

Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector

Popis výsledku anglicky

Detection of the specific sequences of pandemic-causing viral nucleic acids is area attracting numerous scientists worldwide. In this study, we focused on the isolation and detection of oligonucleotide specific sequences of life-threatening viruses (HIV(GAGCAGTGGGAATA), Influenza (TAATAACCATTGGA) and Ebola (ACCTCACTAGAAAA)) by flow injection analysis with electrochemical detection coupled with paramagnetic particles based isolation. All of used viral genomic sequences contained A6 adenine repetition onthe 5 end. This modification enables us to bind them on paramagnetic particle anchoring T25 (MPs-(dT)25). The isolated sequences were subsequently hydrolysed by optimized method using microwaves to obtain single adenines. The obtained samples were detected by microfluidic tool based on stopped flow injection analysis with glassy carbon electrode in the presence of Cu(II)/or CuS nanoparticles to enhance sensitivity and to lower detection limit for adenine. Basic detection parameters as a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

ISSN

1452-3981

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

15

Strana od-do

12628-12642

Kód UT WoS článku

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EID výsledku v databázi Scopus

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