gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F14%3APU111693" target="_blank" >RIV/00216305:26620/14:PU111693 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/14:43873156 RIV/62156489:43210/14:43908680

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

Popis výsledku v původním jazyce

The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The most important tool in the diagnosis of influenza pandemic is timely and right diagnosis. Hence, this study presents synthesis of paramagnetic particles formed by nanomaghemite (gamma-Fe2O3) core, whose surface was functionalized with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTES), able to bind H7N7 influenza virions. Paramagnetic particles show the ability to immobilize virions for direct analysis using ion-exchange chromatography with VIS detection. The presence of H7N7 virions on surface of paramagnetic beads was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry, based upon the mass weight of H7N7 virions, determined before and after establishment of binding. Moreover, using scanning electrochemical microscopy revealed, that binding is provided due to strong charge-charge int

Název v anglickém jazyce

gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

Popis výsledku anglicky

The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The most important tool in the diagnosis of influenza pandemic is timely and right diagnosis. Hence, this study presents synthesis of paramagnetic particles formed by nanomaghemite (gamma-Fe2O3) core, whose surface was functionalized with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTES), able to bind H7N7 influenza virions. Paramagnetic particles show the ability to immobilize virions for direct analysis using ion-exchange chromatography with VIS detection. The presence of H7N7 virions on surface of paramagnetic beads was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry, based upon the mass weight of H7N7 virions, determined before and after establishment of binding. Moreover, using scanning electrochemical microscopy revealed, that binding is provided due to strong charge-charge int

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.4.31.0023" target="_blank" >EE2.4.31.0023: Partnerská síť centra excelentního bionanotechnologického výzkumu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

ISSN

1452-3981

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

12

Strana od-do

3374-3385

Kód UT WoS článku

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EID výsledku v databázi Scopus

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