Dynamic phase differences method for the assessment of cellular dynamic processes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F14%3APU113982" target="_blank" >RIV/00216305:26620/14:PU113982 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ceitec.eu/ac2014-book-of-abstract/f32414" target="_blank" >http://www.ceitec.eu/ac2014-book-of-abstract/f32414</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dynamic phase differences method for the assessment of cellular dynamic processes

Popis výsledku v původním jazyce

Imaging of cellular mass transfers and therefore the traits of live cell behaviour is nowadays a rewarding task in life sciences. As a tool for revealing those dynamic processes, we present a method of dynamic phase differences (DPD). DPD fully exploitsthe quantitative phase images obtained by the Coherence-Controlled Holographic Microscope (CCHM) developed in Brno University of Technology. DPD method is employed for the objective evaluation of the growth, migration and motility of cells, which would not be obvious only from the quantitative phase images. The results can be directly visualised by the 2D colour image, which could lead to identification of typical patterns characterizing the behaviour of cells. The method also provides the opportunity to calculate the transferred mass of the cell over a distance and therefore enables to quantitatively measure the movements of the cell (in picograms).

Název v anglickém jazyce

Dynamic phase differences method for the assessment of cellular dynamic processes

Popis výsledku anglicky

Imaging of cellular mass transfers and therefore the traits of live cell behaviour is nowadays a rewarding task in life sciences. As a tool for revealing those dynamic processes, we present a method of dynamic phase differences (DPD). DPD fully exploitsthe quantitative phase images obtained by the Coherence-Controlled Holographic Microscope (CCHM) developed in Brno University of Technology. DPD method is employed for the objective evaluation of the growth, migration and motility of cells, which would not be obvious only from the quantitative phase images. The results can be directly visualised by the 2D colour image, which could lead to identification of typical patterns characterizing the behaviour of cells. The method also provides the opportunity to calculate the transferred mass of the cell over a distance and therefore enables to quantitatively measure the movements of the cell (in picograms).

Druh

O - Ostatní výsledky

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů