Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU113981" target="_blank" >RIV/00216305:26620/15:PU113981 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.focusonmicroscopy.org/2015/PDF/345_Strbkova.pdf" target="_blank" >http://www.focusonmicroscopy.org/2015/PDF/345_Strbkova.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy

Popis výsledku v původním jazyce

The understanding of the surface-cell interaction plays important role for the biomaterials development and bioengineering. We present the Coherence-Controlled Holographic Microscope (CCHM) built in Brno University of Technology as a tool for a reliable assessment of the biocompatibility of the amine-rich coatings. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of the amine layers properties on cell adhesion need to be further investigated. Imaging and assessment of surface-cell interaction is an arduous task, since the cells are weakly scattering and absorbing specimens. The CCHM enables to acquire speckle-free optically-sectioned quantitative phase images of live cells in high contrast without using any labels. The phase image contains quantitative information, from which valuable parameters directly related to the cell mass can be obtained. Based on those parameters, the biocompatibility of the surfaces is evaluated. The stable amine-rich

Název v anglickém jazyce

Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy

Popis výsledku anglicky

The understanding of the surface-cell interaction plays important role for the biomaterials development and bioengineering. We present the Coherence-Controlled Holographic Microscope (CCHM) built in Brno University of Technology as a tool for a reliable assessment of the biocompatibility of the amine-rich coatings. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of the amine layers properties on cell adhesion need to be further investigated. Imaging and assessment of surface-cell interaction is an arduous task, since the cells are weakly scattering and absorbing specimens. The CCHM enables to acquire speckle-free optically-sectioned quantitative phase images of live cells in high contrast without using any labels. The phase image contains quantitative information, from which valuable parameters directly related to the cell mass can be obtained. Based on those parameters, the biocompatibility of the surfaces is evaluated. The stable amine-rich

Druh

O - Ostatní výsledky

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů