Nanoparticles-Modified Glassy Carbon Electrode in Microflow Arrangement for Analysis of Nucleic Acids Containing Repeated Adenine Sequences

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU126957" target="_blank" >RIV/00216305:26620/15:PU126957 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanoparticles-Modified Glassy Carbon Electrode in Microflow Arrangement for Analysis of Nucleic Acids Containing Repeated Adenine Sequences

Popis výsledku v původním jazyce

In this study we have used the microfluidic tool with a glassy carbon electrode modified with CuS nanoparticles for adenine determination using differential pulse voltammetry. The optimal parameters for adenine detection were the initial potential 0 V, and potential 1.8 V, step potential 0.015 V, amplitude 0.1 V, scan rate 0.07 V/s, pulse period 200 s and equilibration time 20 s. Prior the analysis the glassy carbon electrode ( GCE) was modified by CuS nanoparticles. The optimal conditions for GCE modification were estimated as deposition potential -0.4 V for 200 s in the presence of 0.1M KCl solution containing 200 mM CuS nanoparticles. Subsequently the cyclic voltammetry was carried out in 0.1M NaOH solution repetitively for 20 cycles under potentials ranging from -0.5 to 0.3 V at 100 mV/s. The limit of detection (3 S/N) was estimated as 70 pg/cell volume. The method was applied for the analysis of oligonucleotides containing repetitions of adenine. We have found the dependence between number of adenine molecules in oligonucleotide and the electrochemical signal. This method might be useful for analysis of DNA sequences.

Název v anglickém jazyce

Nanoparticles-Modified Glassy Carbon Electrode in Microflow Arrangement for Analysis of Nucleic Acids Containing Repeated Adenine Sequences

Popis výsledku anglicky

In this study we have used the microfluidic tool with a glassy carbon electrode modified with CuS nanoparticles for adenine determination using differential pulse voltammetry. The optimal parameters for adenine detection were the initial potential 0 V, and potential 1.8 V, step potential 0.015 V, amplitude 0.1 V, scan rate 0.07 V/s, pulse period 200 s and equilibration time 20 s. Prior the analysis the glassy carbon electrode ( GCE) was modified by CuS nanoparticles. The optimal conditions for GCE modification were estimated as deposition potential -0.4 V for 200 s in the presence of 0.1M KCl solution containing 200 mM CuS nanoparticles. Subsequently the cyclic voltammetry was carried out in 0.1M NaOH solution repetitively for 20 cycles under potentials ranging from -0.5 to 0.3 V at 100 mV/s. The limit of detection (3 S/N) was estimated as 70 pg/cell volume. The method was applied for the analysis of oligonucleotides containing repetitions of adenine. We have found the dependence between number of adenine molecules in oligonucleotide and the electrochemical signal. This method might be useful for analysis of DNA sequences.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GAP102%2F11%2F1068" target="_blank" >GAP102/11/1068: Nano-elektro-bio-nástroje pro biochemické a molekulárně-biologické studie eukaryotických buněk (NanoBioTECell)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemické listy

ISSN

0009-2770

e-ISSN

1213-7103

Svazek periodika

109

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

298-304

Kód UT WoS článku

000354722700008

EID výsledku v databázi Scopus

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