Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU127018" target="_blank" >RIV/00216305:26620/15:PU127018 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aca.2015.02.044" target="_blank" >http://dx.doi.org/10.1016/j.aca.2015.02.044</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2015.02.044" target="_blank" >10.1016/j.aca.2015.02.044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Popis výsledku v původním jazyce

In this work, a new type of miniaturized fibre-coupled solid-state light source is demonstrated as an excitation source for fluorescence detection in capillary electrophoresis. It is based on a parabolically shaped micro-light emitting diode (mu-LED) array with a custom band-pass optical interference filter (IF) deposited at the back of the LED substrate. The GaN mu-LED array consisted of 270 individual mu-LED elements with a peak emission at 470 nm, each about 14 mm in diameter and operated as a single unit. Light was extracted through the transparent substrate material, and coupled to an optical fibre (OF, 400 mm in diameter, numerical aperture NA = 0.37), to form an integrated mu-LED-IF-OF light source component. This packaged mu-LED-IF-OF light source emitted approximately 225 mu W of optical power at a bias current of 20 mA. The bandpass IF filter was designed to reduce undesirable LED light emissions in the wavelength range above 490 nm. Devices with and without IF were compared in terms of the optical power output, spectral characteristics as well as LOD values. While the IF consisted of only 7.5 pairs (15 layers) of SiO2/HfO2 layers, it resulted in an improvement of the baseline noise as well as the detection limit measured using fluorescein as test analyte, both by approximately one order of magnitude, with a LOD of 1 x 10(-8) mol L-1 obtained under optimised conditions. The mu-LED-IF-OF light source was then demonstrated for use in capillary electrophoresis with fluorimetric detection. The limits of detection obtained by this device were compared to those obtained with a commercial fibre coupled LED device.

Název v anglickém jazyce

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Popis výsledku anglicky

In this work, a new type of miniaturized fibre-coupled solid-state light source is demonstrated as an excitation source for fluorescence detection in capillary electrophoresis. It is based on a parabolically shaped micro-light emitting diode (mu-LED) array with a custom band-pass optical interference filter (IF) deposited at the back of the LED substrate. The GaN mu-LED array consisted of 270 individual mu-LED elements with a peak emission at 470 nm, each about 14 mm in diameter and operated as a single unit. Light was extracted through the transparent substrate material, and coupled to an optical fibre (OF, 400 mm in diameter, numerical aperture NA = 0.37), to form an integrated mu-LED-IF-OF light source component. This packaged mu-LED-IF-OF light source emitted approximately 225 mu W of optical power at a bias current of 20 mA. The bandpass IF filter was designed to reduce undesirable LED light emissions in the wavelength range above 490 nm. Devices with and without IF were compared in terms of the optical power output, spectral characteristics as well as LOD values. While the IF consisted of only 7.5 pairs (15 layers) of SiO2/HfO2 layers, it resulted in an improvement of the baseline noise as well as the detection limit measured using fluorescein as test analyte, both by approximately one order of magnitude, with a LOD of 1 x 10(-8) mol L-1 obtained under optimised conditions. The mu-LED-IF-OF light source was then demonstrated for use in capillary electrophoresis with fluorimetric detection. The limits of detection obtained by this device were compared to those obtained with a commercial fibre coupled LED device.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ANALYTICA CHIMICA ACTA

ISSN

0003-2670

e-ISSN

1873-4324

Svazek periodika

871

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

85-92

Kód UT WoS článku

000352204100011

EID výsledku v databázi Scopus

2-s2.0-84926407020