Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F16%3APU122797" target="_blank" >RIV/00216305:26620/16:PU122797 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging

Popis výsledku v původním jazyce

Nanoparticle visualization has extensive potential for their utilization in targeted drug delivery tracking through wide range of biological environments. In this regard, quantum dot nanoparticles may represent a suitable option as they are characterized by very good fluorescent properties. This study is focused on application of quantum dot nanoparticles for fluorescence imaging of plants. A series of in vivo experiments was carried out on leaves of sunflower plant (Helianthus annuus). Leaves were immersed in water solution of CdTe and CdTe/ZnS quantum dots and were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, Massachusets, USA). Captured images were further processed and the intensity of fluorescence analyzed by Bruker Molecular Imaging Software. Fluorescence imaging of plants encounters difficulties due to autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophyll. How

Název v anglickém jazyce

Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging

Popis výsledku anglicky

Nanoparticle visualization has extensive potential for their utilization in targeted drug delivery tracking through wide range of biological environments. In this regard, quantum dot nanoparticles may represent a suitable option as they are characterized by very good fluorescent properties. This study is focused on application of quantum dot nanoparticles for fluorescence imaging of plants. A series of in vivo experiments was carried out on leaves of sunflower plant (Helianthus annuus). Leaves were immersed in water solution of CdTe and CdTe/ZnS quantum dots and were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, Massachusets, USA). Captured images were further processed and the intensity of fluorescence analyzed by Bruker Molecular Imaging Software. Fluorescence imaging of plants encounters difficulties due to autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophyll. How

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů