Dual-polarization interference microscopy for advanced quantification of phase associated with the image field

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F18%3APU126352" target="_blank" >RIV/00216305:26620/18:PU126352 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/18:73587161

Výsledek na webu

<a href="https://www.osapublishing.org/ol/abstract.cfm?uri=ol-43-3-427" target="_blank" >https://www.osapublishing.org/ol/abstract.cfm?uri=ol-43-3-427</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1364/OL.43.000427" target="_blank" >10.1364/OL.43.000427</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dual-polarization interference microscopy for advanced quantification of phase associated with the image field

Popis výsledku v původním jazyce

A new concept of dual-polarization spatial light interference microscopy (DPSLIM) is proposed and demonstrated experimentally. The method works with two orthogonally polarized modes in which signal and reference waves are combined to realize the polarization-sensitive phase-shifting, thus allowing advanced reconstruction of the phase associated with the image field. The image phase is reconstructed directly from four polarization encoded interference records by a single step processing. This is a progress compared with common methods, in which the phase of the image field is reconstructed using the optical path difference and the amplitudes of interfering waves, which are calculated in multiple-step processing of the records. The DPSLIM is implemented in a common-path configuration using a spatial light modulator, which is connected to a commercial microscope Nikon E200. The optical performance of the method is demonstrated in experiments using both polystyrene microspheres and live LW13K2 cells.

Název v anglickém jazyce

Dual-polarization interference microscopy for advanced quantification of phase associated with the image field

Popis výsledku anglicky

A new concept of dual-polarization spatial light interference microscopy (DPSLIM) is proposed and demonstrated experimentally. The method works with two orthogonally polarized modes in which signal and reference waves are combined to realize the polarization-sensitive phase-shifting, thus allowing advanced reconstruction of the phase associated with the image field. The image phase is reconstructed directly from four polarization encoded interference records by a single step processing. This is a progress compared with common methods, in which the phase of the image field is reconstructed using the optical path difference and the amplitudes of interfering waves, which are calculated in multiple-step processing of the records. The DPSLIM is implemented in a common-path configuration using a spatial light modulator, which is connected to a commercial microscope Nikon E200. The optical performance of the method is demonstrated in experiments using both polystyrene microspheres and live LW13K2 cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Optics Letters

ISSN

0146-9592

e-ISSN

1539-4794

Svazek periodika

43

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

427-430

Kód UT WoS článku

000423776600020

EID výsledku v databázi Scopus

—