Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F18%3APU128665" target="_blank" >RIV/00216305:26620/18:PU128665 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model

Popis výsledku v původním jazyce

The resorbable dermal replacement was made up of two nanostructured biocompatible layers. The lower part (dermis substitution) was prepared from biopolymer porous foam modified with a highly stable fibroblast growth factor 2 (FGF2). The upper part (dermo - epidermal substitution) consisted of a layer of amphiphilic polymeric nanofibers ensuring optimal adhesion to the epidermal graft. The effect of FGF2 concentration on morphology and biological activity of the skin substitutes was optimized in-vitro by colonization of 3T3 line of mouse fibroblasts. As confirmed by Chick Chorioallantoic Membrane assay, stable FGF2 also significantly enhanced neovascularization efficiency of biopolymer substitutes. Regenerative ability of the resorbable bilayer implant enriched with FGF2 was evaluated in a full-skin thickness defect on both small and large animal model (New Zealand rabbit and Large white pig, respectively). Histological and immuno-histological analysis of samples taken on the 14th postoperative day fro

Název v anglickém jazyce

Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model

Popis výsledku anglicky

The resorbable dermal replacement was made up of two nanostructured biocompatible layers. The lower part (dermis substitution) was prepared from biopolymer porous foam modified with a highly stable fibroblast growth factor 2 (FGF2). The upper part (dermo - epidermal substitution) consisted of a layer of amphiphilic polymeric nanofibers ensuring optimal adhesion to the epidermal graft. The effect of FGF2 concentration on morphology and biological activity of the skin substitutes was optimized in-vitro by colonization of 3T3 line of mouse fibroblasts. As confirmed by Chick Chorioallantoic Membrane assay, stable FGF2 also significantly enhanced neovascularization efficiency of biopolymer substitutes. Regenerative ability of the resorbable bilayer implant enriched with FGF2 was evaluated in a full-skin thickness defect on both small and large animal model (New Zealand rabbit and Large white pig, respectively). Histological and immuno-histological analysis of samples taken on the 14th postoperative day fro

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30502 - Other medical science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů