Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU131607" target="_blank" >RIV/00216305:26620/19:PU131607 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/19:43915172
Výsledek na webu
<a href="http://link.springer.com/article/10.1186/s40104-019-0319-8" target="_blank" >http://link.springer.com/article/10.1186/s40104-019-0319-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s40104-019-0319-8" target="_blank" >10.1186/s40104-019-0319-8</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure
Popis výsledku v původním jazyce
Background: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Results: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6mmol/L), followed by Escherichia coli (IC50 0.8-1.5mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2-4.7mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P<0.05) Zn level in liver and kidney of all treated groups was found, nevertheless ZnNPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased (P<0.05) in all zinc groups after 30d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more (P<0.05). Conclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
Název v anglickém jazyce
Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure
Popis výsledku anglicky
Background: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Results: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6mmol/L), followed by Escherichia coli (IC50 0.8-1.5mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2-4.7mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P<0.05) Zn level in liver and kidney of all treated groups was found, nevertheless ZnNPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased (P<0.05) in all zinc groups after 30d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more (P<0.05). Conclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Animal Science and Biotechnology
ISSN
1674-9782
e-ISSN
2049-1891
Svazek periodika
10
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CN - Čínská lidová republika
Počet stran výsledku
12
Strana od-do
1-12
Kód UT WoS článku
000458771600001
EID výsledku v databázi Scopus
—