Tuning LC-MS/MS analysis for identification of peptide extracts from cryosections of porcine lung tissue affected by Actinobacillus pleuropneumoniae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134873" target="_blank" >RIV/00216305:26620/19:PU134873 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/19:43917124

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tuning LC-MS/MS analysis for identification of peptide extracts from cryosections of porcine lung tissue affected by Actinobacillus pleuropneumoniae

Popis výsledku v původním jazyce

The porcine pleuropneumonia belongs to common pig bacterial infection caused by Actinobacillus pleuropneumoniae. This disease is affecting the economy of pig breeding. Therefore, it is important to better understand its effects and to find targeted therapy. To elucidate this infection different approaches can be used. One of them is to monitor the response of organism and detect changed expressions of proteins connected to immune reaction, especially cytokines subgroup called interleukins. Usually, these proteins are detected by enzyme-linked immunosorbent assay (ELISA), but other methods like label-free liquid chromatography connected with tandem mass spectrometry (LCMS/MS) can be used. This study was aimed at adapting/developing a high-performance liquid chromatography-electrospray ionisation quadrupole time-of-flight mass spectrometry (HPLC-ESIQqTOF MS) method for analysing these infection markers after on-tissue digestion and extraction from the surface of porcine lung tissue sections.

Název v anglickém jazyce

Tuning LC-MS/MS analysis for identification of peptide extracts from cryosections of porcine lung tissue affected by Actinobacillus pleuropneumoniae

Popis výsledku anglicky

The porcine pleuropneumonia belongs to common pig bacterial infection caused by Actinobacillus pleuropneumoniae. This disease is affecting the economy of pig breeding. Therefore, it is important to better understand its effects and to find targeted therapy. To elucidate this infection different approaches can be used. One of them is to monitor the response of organism and detect changed expressions of proteins connected to immune reaction, especially cytokines subgroup called interleukins. Usually, these proteins are detected by enzyme-linked immunosorbent assay (ELISA), but other methods like label-free liquid chromatography connected with tandem mass spectrometry (LCMS/MS) can be used. This study was aimed at adapting/developing a high-performance liquid chromatography-electrospray ionisation quadrupole time-of-flight mass spectrometry (HPLC-ESIQqTOF MS) method for analysing these infection markers after on-tissue digestion and extraction from the surface of porcine lung tissue sections.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019 Proceedings of 26th International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

557-562

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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