Novel Ruthenium coordinate compound combined with Schiff base and benzimidazole as a potent antibacterial agent against VRSA and MRSA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134931" target="_blank" >RIV/00216305:26620/19:PU134931 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/19:43917153

Výsledek na webu

<a href="https://mendelnet.cz/artkey/mnt-201901-0126_Novel-Ruthenium-coordinate-compound-combined-with-Schiff-base-and-benzimidazole-as-a-potent-antibacterial-agent.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0126_Novel-Ruthenium-coordinate-compound-combined-with-Schiff-base-and-benzimidazole-as-a-potent-antibacterial-agent.php?back=/magno/mnt/2019/mn1.php?secid=4</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Novel Ruthenium coordinate compound combined with Schiff base and benzimidazole as a potent antibacterial agent against VRSA and MRSA

Popis výsledku v původním jazyce

The rise of antibiotic-resistant strains is an important public health problem and thus the development of an alternative to antibiotics is imminent. The Ruthenium–Schiff base with benzimidazole (RU–S2), a co-ordinate compound is novel and first of its kind to be synthesized in such combination. The aim of the experiment is based on the synthesis of RU–S2 and to study its antibacterial activity against the pathogenic resistant strains of Staphylococcus aureus like Vancomycin-resistant Staphylococcus aureus (VRSA) and Methicillin-resistant Staphylococcus aureus (MRSA). The antibacterial activity was studied using growth curve analysis based on turbidimetry which was confirmed by the fluorescence live/dead cell microscopic imaging of the bacteria after treatment with RU–S2. Lastly, the cytotoxicity test was performed by 3-(4,5-Dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay against human normal and cancer epithelial cell lines to understand the toxic effects of RU-S2 at 160 µg/ml concentration. The concentration 160 µg/ml of RU–S2 was very effective against those resistant strains and also nontoxic in this concentration. Thus RU-S2 can be used as an efficient alternative to antibiotics that have promising antibacterial efficacy against the pathogenic strains with no toxicity and biocompatibility towards human cells

Název v anglickém jazyce

Novel Ruthenium coordinate compound combined with Schiff base and benzimidazole as a potent antibacterial agent against VRSA and MRSA

Popis výsledku anglicky

The rise of antibiotic-resistant strains is an important public health problem and thus the development of an alternative to antibiotics is imminent. The Ruthenium–Schiff base with benzimidazole (RU–S2), a co-ordinate compound is novel and first of its kind to be synthesized in such combination. The aim of the experiment is based on the synthesis of RU–S2 and to study its antibacterial activity against the pathogenic resistant strains of Staphylococcus aureus like Vancomycin-resistant Staphylococcus aureus (VRSA) and Methicillin-resistant Staphylococcus aureus (MRSA). The antibacterial activity was studied using growth curve analysis based on turbidimetry which was confirmed by the fluorescence live/dead cell microscopic imaging of the bacteria after treatment with RU–S2. Lastly, the cytotoxicity test was performed by 3-(4,5-Dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay against human normal and cancer epithelial cell lines to understand the toxic effects of RU-S2 at 160 µg/ml concentration. The concentration 160 µg/ml of RU–S2 was very effective against those resistant strains and also nontoxic in this concentration. Thus RU-S2 can be used as an efficient alternative to antibiotics that have promising antibacterial efficacy against the pathogenic strains with no toxicity and biocompatibility towards human cells

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019 Proceedings of 26th International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

—

e-ISSN

—

Počet stran výsledku

5

Strana od-do

654-658

Název nakladatele

Mendel University in Brno

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

—