Large Scale Fabrication of Ordered Gold Nanoparticle-Epoxy Surface Nanocomposites and Their Application as Label-Free Plasmonic DNA Biosensors
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F20%3APU135432" target="_blank" >RIV/00216305:26620/20:PU135432 - isvavai.cz</a>
Výsledek na webu
<a href="https://pubs.acs.org/doi/10.1021/acsami.9b20907" target="_blank" >https://pubs.acs.org/doi/10.1021/acsami.9b20907</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.9b20907" target="_blank" >10.1021/acsami.9b20907</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Large Scale Fabrication of Ordered Gold Nanoparticle-Epoxy Surface Nanocomposites and Their Application as Label-Free Plasmonic DNA Biosensors
Popis výsledku v původním jazyce
A robust and scalable technology to fabricate ordered gold nanoparticle arrangements on epoxy substrates is presented. The nanoparticles are synthesized by solid-state dewetting on nanobowled aluminum templates, which are prepared by the selective chemical etching of porous anodic alumina (PAA) grown on an aluminum sheet with controlled anodic oxidation. This flexible fabrication technology provides proper control over the nanoparticle size, shape, and interparticle distance over a large surface area (several cm(2)), which enables the fine-tuning and optimization of their plasmonic absorption spectra for LSPR and SERS applications between 535 and 625 nm. The nanoparticles are transferred to the surface of epoxy substrates, which are subsequently selectively etched. The resulting nanomushrooms arrangements consist of ordered epoxy nanopillars with flat, disk-shaped nanoparticles on top, and their bulk refractive index sensitivity is between 83 and 108 nm RIU-1. Label-free DNA detection is successfully demonstrated with the sensors by using a 20 base pair long specific DNA sequence from the parasite Giardia lamblia. A red-shift of 6.6 nm in the LSPR absorbance spectrum was detected after the 2 h hybridization with 1 pM target DNA, and the achievable LOD was around 5 nM. The reported plasmonic sensor is one of the first surface AuNP/polymer nanocomposites ever reported for the successful label-free detection of DNA.
Název v anglickém jazyce
Large Scale Fabrication of Ordered Gold Nanoparticle-Epoxy Surface Nanocomposites and Their Application as Label-Free Plasmonic DNA Biosensors
Popis výsledku anglicky
A robust and scalable technology to fabricate ordered gold nanoparticle arrangements on epoxy substrates is presented. The nanoparticles are synthesized by solid-state dewetting on nanobowled aluminum templates, which are prepared by the selective chemical etching of porous anodic alumina (PAA) grown on an aluminum sheet with controlled anodic oxidation. This flexible fabrication technology provides proper control over the nanoparticle size, shape, and interparticle distance over a large surface area (several cm(2)), which enables the fine-tuning and optimization of their plasmonic absorption spectra for LSPR and SERS applications between 535 and 625 nm. The nanoparticles are transferred to the surface of epoxy substrates, which are subsequently selectively etched. The resulting nanomushrooms arrangements consist of ordered epoxy nanopillars with flat, disk-shaped nanoparticles on top, and their bulk refractive index sensitivity is between 83 and 108 nm RIU-1. Label-free DNA detection is successfully demonstrated with the sensors by using a 20 base pair long specific DNA sequence from the parasite Giardia lamblia. A red-shift of 6.6 nm in the LSPR absorbance spectrum was detected after the 2 h hybridization with 1 pM target DNA, and the achievable LOD was around 5 nM. The reported plasmonic sensor is one of the first surface AuNP/polymer nanocomposites ever reported for the successful label-free detection of DNA.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
21001 - Nano-materials (production and properties)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
ACS APPL MATER INTER
ISSN
1944-8244
e-ISSN
1944-8252
Svazek periodika
12
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
4804-4814
Kód UT WoS článku
000510532000066
EID výsledku v databázi Scopus
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