SiO2-decorated Parylene C micropillars designed to probe cellular force

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU138327" target="_blank" >RIV/00216305:26620/21:PU138327 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/21:PU143988 RIV/00216224:14110/21:00121732

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/admi.202001897" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/admi.202001897</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

SiO2-decorated Parylene C micropillars designed to probe cellular force

Popis výsledku v původním jazyce

Living cells sense and respond to mechanical signals through specific mechanisms that generate traction force. The quantification of cell forces using micropillars can be limited by micropillar stiffness, technological aspects of the fabrication and microcontact printing of proteins. This paper develops the new design of SiO2/Parylene C micropillars with an aspect ratio of 6 and 3.5 and spring constant of 4.7 µN µm-1 and 28 µN µm-1, respectively. The upper part of individual micropillars is coated with a 250 nm layer of SiO2, and the results confirm protein deposition on the micropillars via the SiO2 interface and non-adhesiveness on the micropillars’ sidewalls. The results show an absence of cytotoxicity for micropillar-based substrates and a dependence on its stiffness. Stiffer micropillars enhance cell adhesion and proliferation rate, and a stronger cellular force of ~ 25µN was obtained. The main contribution of SiO2/Parylene C micropillars is the elimination of the step involving the fabrication of PDMS stamp because our array enables covalent binding of proteins via SiO2 chemistry. These micropillars stand on Si wafer and thus, any warping of underlying polymer membrane does not have to be considered. Additionally, SiO2/Parylene C micropillars can broaden the range of stiffer substrates to be probed by cells.

Název v anglickém jazyce

SiO2-decorated Parylene C micropillars designed to probe cellular force

Popis výsledku anglicky

Living cells sense and respond to mechanical signals through specific mechanisms that generate traction force. The quantification of cell forces using micropillars can be limited by micropillar stiffness, technological aspects of the fabrication and microcontact printing of proteins. This paper develops the new design of SiO2/Parylene C micropillars with an aspect ratio of 6 and 3.5 and spring constant of 4.7 µN µm-1 and 28 µN µm-1, respectively. The upper part of individual micropillars is coated with a 250 nm layer of SiO2, and the results confirm protein deposition on the micropillars via the SiO2 interface and non-adhesiveness on the micropillars’ sidewalls. The results show an absence of cytotoxicity for micropillar-based substrates and a dependence on its stiffness. Stiffer micropillars enhance cell adhesion and proliferation rate, and a stronger cellular force of ~ 25µN was obtained. The main contribution of SiO2/Parylene C micropillars is the elimination of the step involving the fabrication of PDMS stamp because our array enables covalent binding of proteins via SiO2 chemistry. These micropillars stand on Si wafer and thus, any warping of underlying polymer membrane does not have to be considered. Additionally, SiO2/Parylene C micropillars can broaden the range of stiffer substrates to be probed by cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GJ19-04270Y" target="_blank" >GJ19-04270Y: Mapování trakční síly buněk v reálném čase</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Materials Interfaces

ISSN

2196-7350

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000611098000001

EID výsledku v databázi Scopus

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