Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU141193" target="_blank" >RIV/00216305:26620/21:PU141193 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/21:43919822 RIV/00216224:14310/21:00123819

Výsledek na webu

<a href="https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection" target="_blank" >https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3752" target="_blank" >10.37904/nanocon.2020.3752</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Popis výsledku v původním jazyce

Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).

Název v anglickém jazyce

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Popis výsledku anglicky

Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2020 CONFERENCE PROCEEDINGS

ISBN

978-80-87294-98-7

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

4

Strana od-do

395-398

Název nakladatele

Tanger

Místo vydání

neuveden

Místo konání akce

Brno, Hotel Voroněž

Datum konání akce

21. 10. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664505500067