Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU141200" target="_blank" >RIV/00216305:26620/21:PU141200 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/21:10428976

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-021-84579-x" target="_blank" >https://www.nature.com/articles/s41598-021-84579-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-021-84579-x" target="_blank" >10.1038/s41598-021-84579-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

Popis výsledku v původním jazyce

Amyloid plaques are small (similar to 50 mu m), highly-dense aggregates of amyloid beta (A beta) protein in brain tissue, supposed to play a key role in pathogenesis of Alzheimer's disease (AD). Plaques' in vivo detection, spatial distribution and quantitative characterization could be an essential marker in diagnostics and evaluation of AD progress. However, current imaging methods in clinics possess substantial limits in sensitivity towards A beta plaques to play a considerable role in AD screening. Contrast enhanced X-ray micro computed tomography (micro CT) is an emerging highly sensitive imaging technique capable of high resolution visualization of rodent brain. In this study we show the absorption based contrast enhanced X-ray micro CT imaging is viable method for detection and 3D analysis of A beta plaques in transgenic rodent models of Alzheimer's disease. Using iodine contrasted brain tissue isolated from the Tg-F344-AD rat model we show the micro CT imaging is capable of precise imaging of A beta plaques, making possible to further analyze various aspects of their 3D spatial distribution and other properties.

Název v anglickém jazyce

Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

Popis výsledku anglicky

Amyloid plaques are small (similar to 50 mu m), highly-dense aggregates of amyloid beta (A beta) protein in brain tissue, supposed to play a key role in pathogenesis of Alzheimer's disease (AD). Plaques' in vivo detection, spatial distribution and quantitative characterization could be an essential marker in diagnostics and evaluation of AD progress. However, current imaging methods in clinics possess substantial limits in sensitivity towards A beta plaques to play a considerable role in AD screening. Contrast enhanced X-ray micro computed tomography (micro CT) is an emerging highly sensitive imaging technique capable of high resolution visualization of rodent brain. In this study we show the absorption based contrast enhanced X-ray micro CT imaging is viable method for detection and 3D analysis of A beta plaques in transgenic rodent models of Alzheimer's disease. Using iodine contrasted brain tissue isolated from the Tg-F344-AD rat model we show the micro CT imaging is capable of precise imaging of A beta plaques, making possible to further analyze various aspects of their 3D spatial distribution and other properties.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10700 - Other natural sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000630512600001

EID výsledku v databázi Scopus

2-s2.0-85102685147