Recent advances of microcalorimetry for studying cellular metabolic heat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU142164" target="_blank" >RIV/00216305:26620/21:PU142164 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S016599362100176X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S016599362100176X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.trac.2021.116353" target="_blank" >10.1016/j.trac.2021.116353</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Recent advances of microcalorimetry for studying cellular metabolic heat

Popis výsledku v původním jazyce

Cellular metabolic heat (Delta P-M) is produced from intracellular reactions, carrying information about particular processes thus indicating the status of organisms. Delta P-M originating from a single cell is at pW level, making its determination based on the calorimetry technique challenging. Microcalorimetry, evolved from original calorimetry, provides an opportunity to look inside the cell by monitoring Delta P-M at single-cell level due to its high resolution. In this review, we discuss the microcalorimetry-based techniques utilized to measure Delta P-M, as well as its extended applications. We first introduce the characterization of microcalorimeters and propose potential methods to improve the performance of these devices. Then we focus on Delta P-M measurements using microcalorimeters both in microorganisms and mammalian cells. We also review the applications of Delta P-M measurements for fundamental metabolism research and drug susceptibility tests. The measurement of Delta P-M at single-cell level is challenging shedding light on cellular processes and enhance our knowledge in this field. (C) 2021 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Recent advances of microcalorimetry for studying cellular metabolic heat

Popis výsledku anglicky

Cellular metabolic heat (Delta P-M) is produced from intracellular reactions, carrying information about particular processes thus indicating the status of organisms. Delta P-M originating from a single cell is at pW level, making its determination based on the calorimetry technique challenging. Microcalorimetry, evolved from original calorimetry, provides an opportunity to look inside the cell by monitoring Delta P-M at single-cell level due to its high resolution. In this review, we discuss the microcalorimetry-based techniques utilized to measure Delta P-M, as well as its extended applications. We first introduce the characterization of microcalorimeters and propose potential methods to improve the performance of these devices. Then we focus on Delta P-M measurements using microcalorimeters both in microorganisms and mammalian cells. We also review the applications of Delta P-M measurements for fundamental metabolism research and drug susceptibility tests. The measurement of Delta P-M at single-cell level is challenging shedding light on cellular processes and enhance our knowledge in this field. (C) 2021 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA17-20716S" target="_blank" >GA17-20716S: Vysoce přesné mapování vnitřní teploty a energetická balance živých buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

ISSN

0165-9936

e-ISSN

1879-3142

Svazek periodika

143

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

„116353-1“-„116353-10“

Kód UT WoS článku

000711411900016

EID výsledku v databázi Scopus

2-s2.0-85108065003