SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F22%3APU145648" target="_blank" >RIV/00216305:26620/22:PU145648 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216275:25310/22:39919120

Výsledek na webu

<a href="https://www.mdpi.com/1420-3049/27/14/4456" target="_blank" >https://www.mdpi.com/1420-3049/27/14/4456</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules27144456" target="_blank" >10.3390/molecules27144456</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

Popis výsledku v původním jazyce

The immunoreactivity or/and stress response can be induced by nanomaterials' different properties, such as size, shape, etc. These effects are, however, not yet fully understood. This study aimed to clarify the effects of SiO2 nanofibers (SiO(2)NFs) on the cellular responses of THP-1-derived macrophage-like cells. The effects of SiO(2)NFs with different lengths on reactive oxygen species (ROS) and pro-inflammatory cytokines TNF-alpha and IL-1 beta in THP-1 cells were evaluated. From the two tested lengths, it was only the L-SiO(2)NFs with a length approximate to 44 +/- 22 mu m that could induce ROS. Compared to this, only S-SiO(2)NFs with a length approximate to 14 +/- 17 mu m could enhance TNF-alpha and IL-1 beta expression. Our results suggested that L-SiO(2)NFs disassembled by THP-1 cells produced ROS and that the inflammatory reaction was induced by the uptake of S-SiO(2)NFs by THP-1 cells. The F-actin staining results indicated that SiO(2)NFs induced cell motility and phagocytosis. There was no difference in cytotoxicity between L- and S-SiO(2)NFs. However, our results suggested that the lengths of SiO(2)NFs induced different cellular responses.

Název v anglickém jazyce

SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

Popis výsledku anglicky

The immunoreactivity or/and stress response can be induced by nanomaterials' different properties, such as size, shape, etc. These effects are, however, not yet fully understood. This study aimed to clarify the effects of SiO2 nanofibers (SiO(2)NFs) on the cellular responses of THP-1-derived macrophage-like cells. The effects of SiO(2)NFs with different lengths on reactive oxygen species (ROS) and pro-inflammatory cytokines TNF-alpha and IL-1 beta in THP-1 cells were evaluated. From the two tested lengths, it was only the L-SiO(2)NFs with a length approximate to 44 +/- 22 mu m that could induce ROS. Compared to this, only S-SiO(2)NFs with a length approximate to 14 +/- 17 mu m could enhance TNF-alpha and IL-1 beta expression. Our results suggested that L-SiO(2)NFs disassembled by THP-1 cells produced ROS and that the inflammatory reaction was induced by the uptake of S-SiO(2)NFs by THP-1 cells. The F-actin staining results indicated that SiO(2)NFs induced cell motility and phagocytosis. There was no difference in cytotoxicity between L- and S-SiO(2)NFs. However, our results suggested that the lengths of SiO(2)NFs induced different cellular responses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Posilování mezioborové spolupráce ve výzkumu nanomateriálů a při studiu jejich účinků na živé organismy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MOLECULES

ISSN

1420-3049

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

„4456“-„“

Kód UT WoS článku

000831360700001

EID výsledku v databázi Scopus

2-s2.0-85135134080