Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F23%3APU148475" target="_blank" >RIV/00216305:26620/23:PU148475 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/23:43922763

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S003991402200981X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S003991402200981X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2022.124185" target="_blank" >10.1016/j.talanta.2022.124185</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots

Popis výsledku v původním jazyce

Microfluidic paper-based analytical devices modified with molecularly imprinted polymers (mu PADs@MIPs) were developed for fluorescent detection of targeted thiols via in situ UV-induced formation of quantum dots (mu PADs@MIPs@QDs). The selectivity enhancement by the MIP layer formed on the filter paper surface was demonstrated for the isolation of L-homocysteine from wine. Followed by the addition of metal precursors solution (Zn/Cd/Cu) and UV irradiation, fluorescent quantum dots were formed thus enabling quantitative detection of the thiol (serving as a QD capping agent). The effect of different semiconductors was investigated to achieve a lower band gap and higher fluorescence intensity. Increasing fluorescence intensity in the presence of thiol groups was obtained for the following precursors mixture composition: ZnCdCu/S > ZnCd/S > ZnCu/S > ZnS. The proposed method has a good relationship between the fluorescence intensity of ZnCdCu/S QDs and L-homocysteine in a linear range from 0.74 to 7.40 mu M with a limit of detection (LOD) and quantification (LOQ) of 0.51 and 1.71 mu M respectively. This method was applied for the determination of L-homocysteine in white wine with RSD under 6.37%.

Název v anglickém jazyce

Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots

Popis výsledku anglicky

Microfluidic paper-based analytical devices modified with molecularly imprinted polymers (mu PADs@MIPs) were developed for fluorescent detection of targeted thiols via in situ UV-induced formation of quantum dots (mu PADs@MIPs@QDs). The selectivity enhancement by the MIP layer formed on the filter paper surface was demonstrated for the isolation of L-homocysteine from wine. Followed by the addition of metal precursors solution (Zn/Cd/Cu) and UV irradiation, fluorescent quantum dots were formed thus enabling quantitative detection of the thiol (serving as a QD capping agent). The effect of different semiconductors was investigated to achieve a lower band gap and higher fluorescence intensity. Increasing fluorescence intensity in the presence of thiol groups was obtained for the following precursors mixture composition: ZnCdCu/S > ZnCd/S > ZnCu/S > ZnS. The proposed method has a good relationship between the fluorescence intensity of ZnCdCu/S QDs and L-homocysteine in a linear range from 0.74 to 7.40 mu M with a limit of detection (LOD) and quantification (LOQ) of 0.51 and 1.71 mu M respectively. This method was applied for the determination of L-homocysteine in white wine with RSD under 6.37%.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10400 - Chemical sciences

Projekt

<a href="/cs/project/GA19-02108S" target="_blank" >GA19-02108S: Paperfluidická přenosná zařizení pro rychlou a nízkonákladovou analýzu bez instrumentálni detekce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TALANTA

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

255

Číslo periodika v rámci svazku

124185

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

„“-„“

Kód UT WoS článku

000922987800001

EID výsledku v databázi Scopus

2-s2.0-85146051133