Open-channel microfluidic device for TiO2NTs@Fe3O4NPs-assisted viral RNA extraction and amplification-free RNA fluorescence status evaluation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU152640" target="_blank" >RIV/00216305:26620/24:PU152640 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/24:10485741 RIV/00216224:14330/24:00139280 RIV/00216208:11150/24:10485741 RIV/00216208:11160/24:10485741 RIV/00216275:25310/24:39921560

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X24016667?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X24016667?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2024.111554" target="_blank" >10.1016/j.microc.2024.111554</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Open-channel microfluidic device for TiO2NTs@Fe3O4NPs-assisted viral RNA extraction and amplification-free RNA fluorescence status evaluation

Popis výsledku v původním jazyce

Most routine viral RNA detection assays available today rely on RNA amplification steps. The sensitivity of such methods comes at the expense of time, cost, and equipment availability. Given the constraints during the COVID-19 pandemic, such as the limited capacity of RNA amplification instruments and the global shortage of isolation kits, there has been a demand for amplification-free assays which should meet POCT criteria: affordable, sensitive, specific, user-friendly, rapid, and equipment-free. This study aims to design and validate a microanalytical system for direct viral RNA detection which meets these criteria. The presented RNA detection assay consists of four consecutive steps: immunocapturing viral particles, viral particles lysis, TiO2NTs@Fe(3)O(4)NPs-assisted RNA extraction, and finally viral RNA detection. The last three steps occur within a microfluidic device with open channels (OC-MFD). The OC-MFD is manufactured from polycarbonate film by unique mechanical replication techniques in a roll-to-roll setup. This method has been experimentally validated to offer the advantages of low-cost and extremely rapid production (1 mil. pieces of OC-MFDs per hour). Two permanent magnets situated under the application/extraction zones enabled to fix magnetically active particles/nanotubes. At first, viral particles are quantitatively captured from the entire sample volume using immunomagnetic particles. The lysed viral particles are transferred to the application zone of OC-MFD, followed by RNA extraction using magnetic TiO2 nanotubes decorated with Fe3O4 nanoparticles (TiO2NTs@Fe(3)O(4)NPs). The effectivity of the nanomaterial to attract viral RNA molecules was evaluated against a standard RT-qPCR-based laboratory test. Equivalence tests were performed with 143 positive and negative SARS-CoV-2 samples. Spearman rank correlation coefficients demonstrated high agreement for both measured biomarker genes (the E gene equaled 0.76, the RdRp gene equaled 0.73, the p-va

Název v anglickém jazyce

Open-channel microfluidic device for TiO2NTs@Fe3O4NPs-assisted viral RNA extraction and amplification-free RNA fluorescence status evaluation

Popis výsledku anglicky

Most routine viral RNA detection assays available today rely on RNA amplification steps. The sensitivity of such methods comes at the expense of time, cost, and equipment availability. Given the constraints during the COVID-19 pandemic, such as the limited capacity of RNA amplification instruments and the global shortage of isolation kits, there has been a demand for amplification-free assays which should meet POCT criteria: affordable, sensitive, specific, user-friendly, rapid, and equipment-free. This study aims to design and validate a microanalytical system for direct viral RNA detection which meets these criteria. The presented RNA detection assay consists of four consecutive steps: immunocapturing viral particles, viral particles lysis, TiO2NTs@Fe(3)O(4)NPs-assisted RNA extraction, and finally viral RNA detection. The last three steps occur within a microfluidic device with open channels (OC-MFD). The OC-MFD is manufactured from polycarbonate film by unique mechanical replication techniques in a roll-to-roll setup. This method has been experimentally validated to offer the advantages of low-cost and extremely rapid production (1 mil. pieces of OC-MFDs per hour). Two permanent magnets situated under the application/extraction zones enabled to fix magnetically active particles/nanotubes. At first, viral particles are quantitatively captured from the entire sample volume using immunomagnetic particles. The lysed viral particles are transferred to the application zone of OC-MFD, followed by RNA extraction using magnetic TiO2 nanotubes decorated with Fe3O4 nanoparticles (TiO2NTs@Fe(3)O(4)NPs). The effectivity of the nanomaterial to attract viral RNA molecules was evaluated against a standard RT-qPCR-based laboratory test. Equivalence tests were performed with 143 positive and negative SARS-CoV-2 samples. Spearman rank correlation coefficients demonstrated high agreement for both measured biomarker genes (the E gene equaled 0.76, the RdRp gene equaled 0.73, the p-va

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10400 - Chemical sciences

Projekt

<a href="/cs/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Posilování mezioborové spolupráce ve výzkumu nanomateriálů a při studiu jejich účinků na živé organismy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

206

Číslo periodika v rámci svazku

111554

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

„“-„“

Kód UT WoS článku

001317526400001

EID výsledku v databázi Scopus

2-s2.0-85203496386