On-demand in situ hydrogen peroxide generation using red light-A new tool for in vitro redox biology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0198688" target="_blank" >RIV/00216305:26620/26:0198688 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0891584925005192?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0891584925005192?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.freeradbiomed.2025.05.244" target="_blank" >10.1016/j.freeradbiomed.2025.05.244</a>

Jazyk výsledku

angličtina

Název v původním jazyce

On-demand in situ hydrogen peroxide generation using red light-A new tool for in vitro redox biology

Popis výsledku v původním jazyce

Hydrogen peroxide (H2O2) is the most stable form of reactive oxygen species involved in essential cellular processes such as redox signaling, metabolic regulation, and stress response. Conventional delivery methods, such as direct addition or chemical precursors, provide limited spatial and temporal control, making it challenging to study its precise physiological or pathological effects. To address this, there is a growing demand for spatiotemporal H2O2 delivery systems in redox biology. Here, we report on our efforts to create devices that photoelectrochemically reduce dissolved oxygen to hydrogen peroxide, while consuming various organic molecules as electron donors. In this study, we introduce a platform for in situ generation of H2O2 using red light-activated devices available in two configurations: a planar version, that can be integrated onto glass slides or cell culture plates, and a microstructured injectable suspension, which can be introduced into an experimental system as needed. The devices are composed of a gold layer and organic semiconductor materials, with thicknesses of less than 100 nm. Upon illumination, the organic semiconductor side facilitates H2O2 formation through an oxygen reduction reaction, while the gold layer simultaneously enables the oxidation of an electron donor present in the surrounding medium. The devices can provide reliable H2O2 production in complex cell culture media and can be easily used in multiwell plates. This system provides a controllable and non-invasive method for localized H2O2 delivery driven by light illumination.

Název v anglickém jazyce

On-demand in situ hydrogen peroxide generation using red light-A new tool for in vitro redox biology

Popis výsledku anglicky

Hydrogen peroxide (H2O2) is the most stable form of reactive oxygen species involved in essential cellular processes such as redox signaling, metabolic regulation, and stress response. Conventional delivery methods, such as direct addition or chemical precursors, provide limited spatial and temporal control, making it challenging to study its precise physiological or pathological effects. To address this, there is a growing demand for spatiotemporal H2O2 delivery systems in redox biology. Here, we report on our efforts to create devices that photoelectrochemically reduce dissolved oxygen to hydrogen peroxide, while consuming various organic molecules as electron donors. In this study, we introduce a platform for in situ generation of H2O2 using red light-activated devices available in two configurations: a planar version, that can be integrated onto glass slides or cell culture plates, and a microstructured injectable suspension, which can be introduced into an experimental system as needed. The devices are composed of a gold layer and organic semiconductor materials, with thicknesses of less than 100 nm. Upon illumination, the organic semiconductor side facilitates H2O2 formation through an oxygen reduction reaction, while the gold layer simultaneously enables the oxidation of an electron donor present in the surrounding medium. The devices can provide reliable H2O2 production in complex cell culture media and can be easily used in multiwell plates. This system provides a controllable and non-invasive method for localized H2O2 delivery driven by light illumination.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů