Tooth-bone attachment tissue is produced by cells with a mixture of odontoblastic and osteoblastic features in reptiles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0200631" target="_blank" >RIV/00216305:26620/26:0200631 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/joa.70059?getft_integrator=clarivate&src=getftr&utm_source=clarivate" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/joa.70059?getft_integrator=clarivate&src=getftr&utm_source=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/joa.70059" target="_blank" >10.1111/joa.70059</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tooth-bone attachment tissue is produced by cells with a mixture of odontoblastic and osteoblastic features in reptiles

Popis výsledku v původním jazyce

Teeth are anchored in the jaw in a highly variable manner across vertebrates. In mammals and crocodiles, the teeth are cushioned inside bony sockets by periodontal ligaments, whereas most squamate reptiles have teeth firmly attached to the jawbone. Here, we analyzed the development of the attachment tissue in the veiled chameleon, a species with firm acrodont tooth attachment, to reveal the cellular processes establishing ankylosis and to determine the cell types contributing to the attachment. The tooth-bearing bones formed pedicles with edges fusing to the dentine via an attachment tissue produced by morphologically distinct cells exhibiting both osteoblastic and odontoblastic features. These cells were RUNX2-positive, suggesting their potential to differentiate into hard-tissue-producing cells. However, in contrast to the osteoblasts of the bony pedicles, tooth-bone interface (TBI) cells expressed elevated levels of Na+-/K+-ATPase and thus resembled odontoblasts. TBI cells were visible only temporarily, and after tooth-bone fusion they were removed by apoptosis and phagocytosis. Dynamic deposition of the hard matrix continued on both sides of the TBI and during the posthatching stages through the participation of osteoblasts. Overall, our findings demonstrate both odontoblast- and osteoblast-like characteristics of cells producing the attachment tissue at the TBI during development in chameleons, highlighting the existence of a transient intermediate cell population, which we call ankyloblasts.

Název v anglickém jazyce

Tooth-bone attachment tissue is produced by cells with a mixture of odontoblastic and osteoblastic features in reptiles

Popis výsledku anglicky

Teeth are anchored in the jaw in a highly variable manner across vertebrates. In mammals and crocodiles, the teeth are cushioned inside bony sockets by periodontal ligaments, whereas most squamate reptiles have teeth firmly attached to the jawbone. Here, we analyzed the development of the attachment tissue in the veiled chameleon, a species with firm acrodont tooth attachment, to reveal the cellular processes establishing ankylosis and to determine the cell types contributing to the attachment. The tooth-bearing bones formed pedicles with edges fusing to the dentine via an attachment tissue produced by morphologically distinct cells exhibiting both osteoblastic and odontoblastic features. These cells were RUNX2-positive, suggesting their potential to differentiate into hard-tissue-producing cells. However, in contrast to the osteoblasts of the bony pedicles, tooth-bone interface (TBI) cells expressed elevated levels of Na+-/K+-ATPase and thus resembled odontoblasts. TBI cells were visible only temporarily, and after tooth-bone fusion they were removed by apoptosis and phagocytosis. Dynamic deposition of the hard matrix continued on both sides of the TBI and during the posthatching stages through the participation of osteoblasts. Overall, our findings demonstrate both odontoblast- and osteoblast-like characteristics of cells producing the attachment tissue at the TBI during development in chameleons, highlighting the existence of a transient intermediate cell population, which we call ankyloblasts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

<a href="/cs/project/LM2023050" target="_blank" >LM2023050: Národní infrastruktura pro biologické a medicínské zobrazování</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of anatomy

ISSN

0021-8782

e-ISSN

1469-7580

Svazek periodika

248

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

251-268

Kód UT WoS článku

001618260100001

EID výsledku v databázi Scopus

2-s2.0-105022646464