Advancing microplastic detection: non-destructive 3D imaging of microplastics in zebrafish using micro x-ray computed tomography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0200845" target="_blank" >RIV/00216305:26620/26:0200845 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Advancing microplastic detection: non-destructive 3D imaging of microplastics in zebrafish using micro x-ray computed tomography

Popis výsledku v původním jazyce

Plastic pollution is a global environmental issue, and microplastics (MPs) pose increasing concerns due to their potential impact on ecosystems and human health. MPs enter organisms through ingestion and inhalation and accumulate in various tissues. Understanding their distribution within biological systems is crucial for assessing their effects, yet current detection methods face significant limitations. Conventional spectroscopic and pyrolytic techniques provide chemical composition data but require extensive sample preparation, leading to a loss of spatial distribution information and increased contamination risks. To address these challenges, this study explores the application of X-ray micro-computed tomography (microCT) as a non-destructive method for detecting MPs in biological tissues. Using zebrafish as a model organism, we demonstrate how microCT enables precise 3D visualization of MP localization without altering tissue integrity. By optimizing scanning parameters and employing contrast-enhancing techniques, we successfully detected polyethylene MPs within fish intestines. Our findings highlight the potential of microCT for tracking MP accumulation in biological samples, offering a complementary approach to conventional methods. MicroCT’s ability to retain spatial information presents new opportunities for studying MP behavior in biological systems. This technique could be further developed for environmental monitoring and biomedical research, providing insights into the potential health risks of MP exposure. Future work will focus on refining detection capabilities for MPs of different sizes, densities, and shapes, expanding the applicability of microCT in microplastic research.

Název v anglickém jazyce

Advancing microplastic detection: non-destructive 3D imaging of microplastics in zebrafish using micro x-ray computed tomography

Popis výsledku anglicky

Plastic pollution is a global environmental issue, and microplastics (MPs) pose increasing concerns due to their potential impact on ecosystems and human health. MPs enter organisms through ingestion and inhalation and accumulate in various tissues. Understanding their distribution within biological systems is crucial for assessing their effects, yet current detection methods face significant limitations. Conventional spectroscopic and pyrolytic techniques provide chemical composition data but require extensive sample preparation, leading to a loss of spatial distribution information and increased contamination risks. To address these challenges, this study explores the application of X-ray micro-computed tomography (microCT) as a non-destructive method for detecting MPs in biological tissues. Using zebrafish as a model organism, we demonstrate how microCT enables precise 3D visualization of MP localization without altering tissue integrity. By optimizing scanning parameters and employing contrast-enhancing techniques, we successfully detected polyethylene MPs within fish intestines. Our findings highlight the potential of microCT for tracking MP accumulation in biological samples, offering a complementary approach to conventional methods. MicroCT’s ability to retain spatial information presents new opportunities for studying MP behavior in biological systems. This technique could be further developed for environmental monitoring and biomedical research, providing insights into the potential health risks of MP exposure. Future work will focus on refining detection capabilities for MPs of different sizes, densities, and shapes, expanding the applicability of microCT in microplastic research.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/GF23-13617L" target="_blank" >GF23-13617L: Vliv mikroplastů na modelové organismy a jejich osud v životním prostředí za použití zobrazovacích a spektroskopických technik</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů