Myrmecia israeliensis as the primary symbiotic microalga in squamulose lichens growing in European and Canary Island terricolous communities

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00228745%3A_____%2F18%3AN0000006" target="_blank" >RIV/00228745:_____/18:N0000006 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/18:10389713

Výsledek na webu

<a href="https://fottea.czechphycology.cz/pdfs/fot/2018/01/06.pdf" target="_blank" >https://fottea.czechphycology.cz/pdfs/fot/2018/01/06.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/fot.2017.022" target="_blank" >10.5507/fot.2017.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Myrmecia israeliensis as the primary symbiotic microalga in squamulose lichens growing in European and Canary Island terricolous communities

Popis výsledku v původním jazyce

Myrmecia israeliensis has been traditionally considered as a green coccoid free–living microalga. This microalga was previously suggested as the primary phycobiont in the lichens Placidium spp., Heteroplacidium spp., and Psora decipiens. However, due to the absence of ITS rDNA sequences (barcode information) published along with these investigations, the symbiotic nature of M. israeliensis might be confirmed by using the DNA barcoding and different microscopic examinations both in the symbiotic state and in culture. The aim of this study was to settle the presence of M. israeliensis as the primary microalga in squamulose lichens growing in terricolous communities (Psora spp., Placidium spp. and Claviscidium spp.) in 32 localities within European and Canary Island ecosystems by using both molecular and ultrastructural techniques. The lichen–forming fungi were identified using ITS rDNA as a barcode, and in the case of P. decipiens specimens, the mycobiont analyses showed an unexpected variability. Phycobiont phylogenetic analyses were made using both chloroplast (LSU rDNA) and nuclear (ITS rDNA) molecular markers. Our results proved that M. israeliensis is the primary symbiotic microalga in all the chosen and analyzed lichens. In addition, fluorescence microscopy, transmission electron microscopy and scanning electron techniques were used to characterize M. israeliensis. Finally, the presence of this microalga in lichen thalli was verified using different microscopic observations. A combination of different techniques, both molecular and microscopic, allowed for the accurate identification of this symbiotic microalga, beforehand mainly known as free living. Here, we suggest the combination of these techniques to prevent incorrect identification in microalgal lichen studies.

Název v anglickém jazyce

Myrmecia israeliensis as the primary symbiotic microalga in squamulose lichens growing in European and Canary Island terricolous communities

Popis výsledku anglicky

Myrmecia israeliensis has been traditionally considered as a green coccoid free–living microalga. This microalga was previously suggested as the primary phycobiont in the lichens Placidium spp., Heteroplacidium spp., and Psora decipiens. However, due to the absence of ITS rDNA sequences (barcode information) published along with these investigations, the symbiotic nature of M. israeliensis might be confirmed by using the DNA barcoding and different microscopic examinations both in the symbiotic state and in culture. The aim of this study was to settle the presence of M. israeliensis as the primary microalga in squamulose lichens growing in terricolous communities (Psora spp., Placidium spp. and Claviscidium spp.) in 32 localities within European and Canary Island ecosystems by using both molecular and ultrastructural techniques. The lichen–forming fungi were identified using ITS rDNA as a barcode, and in the case of P. decipiens specimens, the mycobiont analyses showed an unexpected variability. Phycobiont phylogenetic analyses were made using both chloroplast (LSU rDNA) and nuclear (ITS rDNA) molecular markers. Our results proved that M. israeliensis is the primary symbiotic microalga in all the chosen and analyzed lichens. In addition, fluorescence microscopy, transmission electron microscopy and scanning electron techniques were used to characterize M. israeliensis. Finally, the presence of this microalga in lichen thalli was verified using different microscopic observations. A combination of different techniques, both molecular and microscopic, allowed for the accurate identification of this symbiotic microalga, beforehand mainly known as free living. Here, we suggest the combination of these techniques to prevent incorrect identification in microalgal lichen studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fottea

ISSN

1802-5439

e-ISSN

neuveden

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

72-85

Kód UT WoS článku

000431867200007

EID výsledku v databázi Scopus

—