Retrograde Oxygen Persufflation of Kidney - Experiment on an Animal

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F16%3A10330073" target="_blank" >RIV/00669806:_____/16:10330073 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/16:00303672 RIV/00216208:11140/16:10330073

Výsledek na webu

<a href="http://dx.doi.org/10.21873/invivo.10997" target="_blank" >http://dx.doi.org/10.21873/invivo.10997</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21873/invivo.10997" target="_blank" >10.21873/invivo.10997</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Retrograde Oxygen Persufflation of Kidney - Experiment on an Animal

Popis výsledku v původním jazyce

Aim: There is still a lack of organs for transplantation purposes. In the field of kidney and liver transplantation, one available solution is the use of organs from so-called marginal donors. These donors can be e.g. non-heart-beating donors. In these cases, perfusion and preservation of organs intended for transplantation is generally more difficult. Retrograde oxygen persufflation (ROP) may be a possible solution to this issue. This method is based on retrograde perfusion by oxygen through the renal vein thus reconditioning the organ. Materials and Methods: We operated on 10 animals (porcine models). Ischemic injury of the right kidney was simulated in all animals . In group A (N=5), kidneys were perfused with retrograde oxygen persufflation after explantation . In group B (N=5), kidneys were perfused intrarterially as in usual clinical practice. After perfusion all kidneys were transplanted to the original donor animal. Quality of graft restitution was evaluated by urea level taken from the renal vein and by histopathological analysis after explantation. Results: We found no statistically significant differences between groups A and B in urea levels after transplantation, nor did we find any significant differences in quality of kidney parenchyma restoration between these groups. Conclusion: Retrograde oxygen persufflation is able to protect and restore kidney parenchyma.

Název v anglickém jazyce

Retrograde Oxygen Persufflation of Kidney - Experiment on an Animal

Popis výsledku anglicky

Aim: There is still a lack of organs for transplantation purposes. In the field of kidney and liver transplantation, one available solution is the use of organs from so-called marginal donors. These donors can be e.g. non-heart-beating donors. In these cases, perfusion and preservation of organs intended for transplantation is generally more difficult. Retrograde oxygen persufflation (ROP) may be a possible solution to this issue. This method is based on retrograde perfusion by oxygen through the renal vein thus reconditioning the organ. Materials and Methods: We operated on 10 animals (porcine models). Ischemic injury of the right kidney was simulated in all animals . In group A (N=5), kidneys were perfused with retrograde oxygen persufflation after explantation . In group B (N=5), kidneys were perfused intrarterially as in usual clinical practice. After perfusion all kidneys were transplanted to the original donor animal. Quality of graft restitution was evaluated by urea level taken from the renal vein and by histopathological analysis after explantation. Results: We found no statistically significant differences between groups A and B in urea levels after transplantation, nor did we find any significant differences in quality of kidney parenchyma restoration between these groups. Conclusion: Retrograde oxygen persufflation is able to protect and restore kidney parenchyma.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FJ - Chirurgie včetně transplantologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

In Vivo

ISSN

0258-851X

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

5

Strana od-do

801-805

Kód UT WoS článku

000392926200011

EID výsledku v databázi Scopus

2-s2.0-84994385696