The method of long-term catheterization of the vena jugularis in pigs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F19%3A10398698" target="_blank" >RIV/00669806:_____/19:10398698 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/19:10398698

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=DJpsJLsEY~" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=DJpsJLsEY~</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vascn.2019.106584" target="_blank" >10.1016/j.vascn.2019.106584</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The method of long-term catheterization of the vena jugularis in pigs

Popis výsledku v původním jazyce

Introduction: The pig is one of the most valuable in vivo models in biomedical research, however with only a few well-accessible veins suitable for venipuncture. Moreover, most of the known methods of blood collection are suitable only for a limited time period. The aim of the study was to verify an improved method of long-term catheterization of the jugular vein in pigs. Methods: A 420 mm polyurethane catheter 16G tube was surgically inserted using the Seldinger technique. The part of the tube that was not inserted into the vein was threaded through a subcutaneously introduced trocar into the occipital area, where it was well accessible and well protected from damage. The catheters were flushed with sterile 0.9% saline solution and locked with 4% citrate between frequent blood samplings, or with 30% citrate at intervals of 1-2-days. Once a week, the catheters were locked with 4% citrate containing taurolidine for 24 h in order to prevent infection. The method was verified in 14 pigs. Results: The catheters were fully functional for up to 11 weeks and no infection or thrombus was observed. Discussion: This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling.

Název v anglickém jazyce

The method of long-term catheterization of the vena jugularis in pigs

Popis výsledku anglicky

Introduction: The pig is one of the most valuable in vivo models in biomedical research, however with only a few well-accessible veins suitable for venipuncture. Moreover, most of the known methods of blood collection are suitable only for a limited time period. The aim of the study was to verify an improved method of long-term catheterization of the jugular vein in pigs. Methods: A 420 mm polyurethane catheter 16G tube was surgically inserted using the Seldinger technique. The part of the tube that was not inserted into the vein was threaded through a subcutaneously introduced trocar into the occipital area, where it was well accessible and well protected from damage. The catheters were flushed with sterile 0.9% saline solution and locked with 4% citrate between frequent blood samplings, or with 30% citrate at intervals of 1-2-days. Once a week, the catheters were locked with 4% citrate containing taurolidine for 24 h in order to prevent infection. The method was verified in 14 pigs. Results: The catheters were fully functional for up to 11 weeks and no infection or thrombus was observed. Discussion: This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

<a href="/cs/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmacological and Toxicological Methods

ISSN

1056-8719

e-ISSN

—

Svazek periodika

98

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

106584

Kód UT WoS článku

000480616400011

EID výsledku v databázi Scopus

2-s2.0-85067968361