Protein expression and localization of ABC transporters in pancreatic adenocarcinoma: prognostic role of ABCC8

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F23%3A10469365" target="_blank" >RIV/00669806:_____/23:10469365 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/23:43926107 RIV/00216208:11140/23:10469365 RIV/61989592:15110/23:73621204

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=uyDTqvsQm-" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=uyDTqvsQm-</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pan.2023.10.008" target="_blank" >10.1016/j.pan.2023.10.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protein expression and localization of ABC transporters in pancreatic adenocarcinoma: prognostic role of ABCC8

Popis výsledku v původním jazyce

Background: ATP-binding cassette (ABC) transporters translocate various substances across cellular membranes. Their deregulation may cause cancer drug resistance or perturbations in the supply of building blocks for cancer cells and modify patients&apos; prognosis. This study investigated protein expression and cellular localization of the previously suggested putative prognostic biomarkers - ABCB2/TAP1, ABCC7/CFTR, ABCC8/SUR1, and ABCD4 in patients with pancreatic ductal adenocarcinoma (PDAC).Methods: Protein expression and localization were assessed by immunohistochemistry in formalin-fixed paraffin-embedded primary tumor tissue blocks of 61 PDAC patients and associated with clinical data and the survival of patients.Results: No CFTR protein expression was observed in PDAC, while TAP1 and ABCC8 were expressed predominantly in the cytoplasm of tumor cells. Most samples (81%) had detectable both membranous and cytoplasmic ABCD4 staining and 42% had ABCD4 expressed in the apical orientation. Negative membranous ABCD4 staining was significantly more frequent in advanced stage III or IV tumors (p=0.022). Small or medium counts of individual ABCC8-positive cells in the stroma surrounding tumor tubules were also more often found in stage III or IV (p=0.044). Patients with moderate or strong ABCC8 cytoplasmic staining intensity in tumor cells had a 3.5-fold higher risk of disease progression than those with weak staining (p=0.002).Conclusions: The study shows for the first time that the cytoplasmic ABCC8 protein expression has prognostic value in PDAC.

Název v anglickém jazyce

Protein expression and localization of ABC transporters in pancreatic adenocarcinoma: prognostic role of ABCC8

Popis výsledku anglicky

Background: ATP-binding cassette (ABC) transporters translocate various substances across cellular membranes. Their deregulation may cause cancer drug resistance or perturbations in the supply of building blocks for cancer cells and modify patients&apos; prognosis. This study investigated protein expression and cellular localization of the previously suggested putative prognostic biomarkers - ABCB2/TAP1, ABCC7/CFTR, ABCC8/SUR1, and ABCD4 in patients with pancreatic ductal adenocarcinoma (PDAC).Methods: Protein expression and localization were assessed by immunohistochemistry in formalin-fixed paraffin-embedded primary tumor tissue blocks of 61 PDAC patients and associated with clinical data and the survival of patients.Results: No CFTR protein expression was observed in PDAC, while TAP1 and ABCC8 were expressed predominantly in the cytoplasm of tumor cells. Most samples (81%) had detectable both membranous and cytoplasmic ABCD4 staining and 42% had ABCD4 expressed in the apical orientation. Negative membranous ABCD4 staining was significantly more frequent in advanced stage III or IV tumors (p=0.022). Small or medium counts of individual ABCC8-positive cells in the stroma surrounding tumor tubules were also more often found in stage III or IV (p=0.044). Patients with moderate or strong ABCC8 cytoplasmic staining intensity in tumor cells had a 3.5-fold higher risk of disease progression than those with weak staining (p=0.002).Conclusions: The study shows for the first time that the cytoplasmic ABCC8 protein expression has prognostic value in PDAC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30219 - Gastroenterology and hepatology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pancreatology

ISSN

1424-3903

e-ISSN

1424-3911

Svazek periodika

23

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

978-987

Kód UT WoS článku

001136921700001

EID výsledku v databázi Scopus

2-s2.0-85173838860