Nephrotic syndrome sera induce different transcriptomes in podocytes based on the steroid response

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F24%3A10475500" target="_blank" >RIV/00669806:_____/24:10475500 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/24:10475500 RIV/00216208:11140/24:10475500 RIV/00064203:_____/24:10475500

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=azoU9SXZAR" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=azoU9SXZAR</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14814/phy2.15932" target="_blank" >10.14814/phy2.15932</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nephrotic syndrome sera induce different transcriptomes in podocytes based on the steroid response

Popis výsledku v původním jazyce

As the molecular mechanism of nephrotic syndrome remains largely undiscovered, patients continue to be exposed to the pros and cons of uniform glucocorticoid treatment. We explored whether the exposure of in vitro-cultivated podocytes to sera from children with steroid-sensitive or steroid-resistant nephrotic syndrome induces differences in gene expression profiles, which could help to elucidate the pathogenesis of the steroid response. Human immortalized podocytes were cultivated with patient sera for 3 days. After cell lysis, RNA extraction, 3&apos;-mRNA libraries were prepared and sequenced. There were 34 significantly upregulated and 14 downregulated genes (fold difference &lt;0.5 and &gt;2.0, respectively, and false discovery rate-corrected p&lt;0.05) and 22 significantly upregulated and 6 downregulated pathways (false discovery rate-corrected p&lt;0.01) in the steroidsensitive (n=9) versus steroid-resistant group (n=4). The observed pathways included upregulated redox reactions, DNA repair, mitosis, protein translations and downregulated cholesterol biosyntesis. Sera from children with nephrotic syndrome induce disease subtype-specific transcriptome changes in human podocytes in vitro. However, further exploration of a larger cohort is needed to verify whether clinically distinct types of nephrotic syndrome or disease activity may be differentiated by specific transcriptomic profiles and whether this informations may help to elucidate the pathogenesis of the steroid response.

Název v anglickém jazyce

Nephrotic syndrome sera induce different transcriptomes in podocytes based on the steroid response

Popis výsledku anglicky

As the molecular mechanism of nephrotic syndrome remains largely undiscovered, patients continue to be exposed to the pros and cons of uniform glucocorticoid treatment. We explored whether the exposure of in vitro-cultivated podocytes to sera from children with steroid-sensitive or steroid-resistant nephrotic syndrome induces differences in gene expression profiles, which could help to elucidate the pathogenesis of the steroid response. Human immortalized podocytes were cultivated with patient sera for 3 days. After cell lysis, RNA extraction, 3&apos;-mRNA libraries were prepared and sequenced. There were 34 significantly upregulated and 14 downregulated genes (fold difference &lt;0.5 and &gt;2.0, respectively, and false discovery rate-corrected p&lt;0.05) and 22 significantly upregulated and 6 downregulated pathways (false discovery rate-corrected p&lt;0.01) in the steroidsensitive (n=9) versus steroid-resistant group (n=4). The observed pathways included upregulated redox reactions, DNA repair, mitosis, protein translations and downregulated cholesterol biosyntesis. Sera from children with nephrotic syndrome induce disease subtype-specific transcriptome changes in human podocytes in vitro. However, further exploration of a larger cohort is needed to verify whether clinically distinct types of nephrotic syndrome or disease activity may be differentiated by specific transcriptomic profiles and whether this informations may help to elucidate the pathogenesis of the steroid response.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30209 - Paediatrics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Reports

ISSN

2051-817X

e-ISSN

2051-817X

Svazek periodika

12

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

e15932

Kód UT WoS článku

001156426900001

EID výsledku v databázi Scopus

2-s2.0-85183729748