Fast and easy simultaneous determination of riboflavin, folic acid, all-trans-retinol and alpha-tocopherol in human serum by LC/MS/MS for bariatric patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F24%3AE0111096" target="_blank" >RIV/00843989:_____/24:E0111096 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/chromsci/article-abstract/62/8/767/7688386?redirectedFrom=fulltext&login=true" target="_blank" >https://academic.oup.com/chromsci/article-abstract/62/8/767/7688386?redirectedFrom=fulltext&login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/chromsci/bmae035" target="_blank" >10.1093/chromsci/bmae035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fast and easy simultaneous determination of riboflavin, folic acid, all-trans-retinol and alpha-tocopherol in human serum by LC/MS/MS for bariatric patients

Popis výsledku v původním jazyce

The aim of this study was to develop and validate methods for the determination of vitamins B2, B9, E and A in serum using liquid chromatography with mass spectrometry (MS) detection. Vitamin analysis was performed using an ultra performance liquid chromatography combined with tandem MS. The compounds were separated on a BEH C18 RP column (2.1 × 100 mm, 1.7 µm) using a gradient elution with an analysis time of 10 min. Sample preparation included protein precipitation with ethanol. The concentration range in human serum was as follows: riboflavin 5-1000 nmol/L, folic acid 2.5-250 nmol/L, ?-tocopherol 0.5-100 µmol/L and all-trans-retinol 25-2500 nmol/L. Accuracy and precision were validated according to Food and Drug Administration guidelines, with coefficients of variation ranging from 3.1-11.7% and recoveries from 94.4-107.5%. Routine monitoring of the complex range of vitamins in bariatric medicine is still not common. This is despite the fact that patients are at risk for glitch deficits, especially of a neurological nature. An analytical method that allows for the complex measurement of both water-soluble and fat-soluble vitamins is important and necessary for the clinical monitoring of bariatric patients. The method we have described could benefit both clinical practice and nutritional research.

Název v anglickém jazyce

Fast and easy simultaneous determination of riboflavin, folic acid, all-trans-retinol and alpha-tocopherol in human serum by LC/MS/MS for bariatric patients

Popis výsledku anglicky

The aim of this study was to develop and validate methods for the determination of vitamins B2, B9, E and A in serum using liquid chromatography with mass spectrometry (MS) detection. Vitamin analysis was performed using an ultra performance liquid chromatography combined with tandem MS. The compounds were separated on a BEH C18 RP column (2.1 × 100 mm, 1.7 µm) using a gradient elution with an analysis time of 10 min. Sample preparation included protein precipitation with ethanol. The concentration range in human serum was as follows: riboflavin 5-1000 nmol/L, folic acid 2.5-250 nmol/L, ?-tocopherol 0.5-100 µmol/L and all-trans-retinol 25-2500 nmol/L. Accuracy and precision were validated according to Food and Drug Administration guidelines, with coefficients of variation ranging from 3.1-11.7% and recoveries from 94.4-107.5%. Routine monitoring of the complex range of vitamins in bariatric medicine is still not common. This is despite the fact that patients are at risk for glitch deficits, especially of a neurological nature. An analytical method that allows for the complex measurement of both water-soluble and fat-soluble vitamins is important and necessary for the clinical monitoring of bariatric patients. The method we have described could benefit both clinical practice and nutritional research.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30107 - Medicinal chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of chromatographic science

ISSN

0021-9665

e-ISSN

1945-239X

Svazek periodika

62

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

767-775

Kód UT WoS článku

001240153300001

EID výsledku v databázi Scopus

2-s2.0-85205603793