First White Rhino Embryo Produced in vitro Enhances Chances for Survival of the Northern White Rhinoceros.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F08568901%3A_____%2F18%3AN0000001" target="_blank" >RIV/08568901:_____/18:N0000001 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

First White Rhino Embryo Produced in vitro Enhances Chances for Survival of the Northern White Rhinoceros.

Popis výsledku v původním jazyce

The Northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal' in existence. Only two females survive - they belong to a European zoo, but reside in Kenya and both are now infertile. The only way to create a self-sustaining population of the NWR is to use Assisted Reproductive Technologies (ART) combined with emerging stem cell technologies. Our consortium achieved the first development of a white rhino embryo to the blastocyst stage in vitro. Samples collected from European zoos resulted in both pure-bred Souther white rhinoceros (SWR) and hybrid femaleSWR * maleNWR blastocysts (pre-implantation embryos). After collection, the oocytes from SWR females were transported to a specialised lab in a shipping incubator that kept temperature of 22 °C for up to 48 h. In the lab, the cocytes were matured and fertilised by intracytoplasmic sperm injection (ICSI) with thawed semen, in some cases even with NWR semen from males that deceased vears ago. Several embryosinjection (ICSI) with thawed semen, in some cases even with NWR semen from males that deceased years ago. Several embryos are now cryopreserved for future transfer into surrogate mothers. The development of a white rhino embryo opens up the potential to bring back NWR from the brink of extinction. This would be achieved by adopting the procedure to oocytes to be collected from the last two living NWR females. Female SWR could act as surrogate mothers. The authors would like to thank to white rhino EEP coordinator Lars Versteege as well as to all zoos that allowed the team to harvest oocytes from their southern white rhino females: Zoo Budapest, Zoo Chorzów, Zoo Dvur Králové, Longleat Safari Park, Montpellier Zoo, Zoo Pairi Daiza, Zoo Poznan, Zoo Salzburg, Zoo Schwerin and Zoo Thoiry. Reference: Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C (2018): Embryos and embryonic stem cells from the white rhinoceros. Nature Communications 9, Article Number: 2589. DOl: 10.1038/s41467-018-04959-2

Název v anglickém jazyce

First White Rhino Embryo Produced in vitro Enhances Chances for Survival of the Northern White Rhinoceros.

Popis výsledku anglicky

The Northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal' in existence. Only two females survive - they belong to a European zoo, but reside in Kenya and both are now infertile. The only way to create a self-sustaining population of the NWR is to use Assisted Reproductive Technologies (ART) combined with emerging stem cell technologies. Our consortium achieved the first development of a white rhino embryo to the blastocyst stage in vitro. Samples collected from European zoos resulted in both pure-bred Souther white rhinoceros (SWR) and hybrid femaleSWR * maleNWR blastocysts (pre-implantation embryos). After collection, the oocytes from SWR females were transported to a specialised lab in a shipping incubator that kept temperature of 22 °C for up to 48 h. In the lab, the cocytes were matured and fertilised by intracytoplasmic sperm injection (ICSI) with thawed semen, in some cases even with NWR semen from males that deceased vears ago. Several embryosinjection (ICSI) with thawed semen, in some cases even with NWR semen from males that deceased years ago. Several embryos are now cryopreserved for future transfer into surrogate mothers. The development of a white rhino embryo opens up the potential to bring back NWR from the brink of extinction. This would be achieved by adopting the procedure to oocytes to be collected from the last two living NWR females. Female SWR could act as surrogate mothers. The authors would like to thank to white rhino EEP coordinator Lars Versteege as well as to all zoos that allowed the team to harvest oocytes from their southern white rhino females: Zoo Budapest, Zoo Chorzów, Zoo Dvur Králové, Longleat Safari Park, Montpellier Zoo, Zoo Pairi Daiza, Zoo Poznan, Zoo Salzburg, Zoo Schwerin and Zoo Thoiry. Reference: Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C (2018): Embryos and embryonic stem cells from the white rhinoceros. Nature Communications 9, Article Number: 2589. DOl: 10.1038/s41467-018-04959-2

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů