Maintenance of Vaccinium gene resources in actively growing in vitro cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F09%3A0R000907" target="_blank" >RIV/25271121:_____/09:0R000907 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Maintenance of Vaccinium gene resources in actively growing in vitro cultures

Popis výsledku v původním jazyce

The possibility of establishment of in vitro duplicate collections of selected highbush blueberry and lingonberry cultivars was investigated. Genotypes of highbush blueberry (Blueray, Bluecrop and Berkeley) and one genotype of lingonberry (Linnea) were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson?s medium (AN) and McCown medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested for repeated subcultures. Based on the obtained results, WPM proved to be more suitable for cultivation of selected cultivars in in vitro cultures. Within the same range of zeatin concentration, the four genotypes gave higher multiplication rates on WPM medium. Acrossall experiments, the highest multiplication rate (4.8) was obtained for ´Berkeley´ on WPM medium with the highest concentration 2 mg/l of zeatin. After three months of storage under normal growing conditions, the survival rate was very h

Název v anglickém jazyce

Maintenance of Vaccinium gene resources in actively growing in vitro cultures

Popis výsledku anglicky

The possibility of establishment of in vitro duplicate collections of selected highbush blueberry and lingonberry cultivars was investigated. Genotypes of highbush blueberry (Blueray, Bluecrop and Berkeley) and one genotype of lingonberry (Linnea) were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson?s medium (AN) and McCown medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested for repeated subcultures. Based on the obtained results, WPM proved to be more suitable for cultivation of selected cultivars in in vitro cultures. Within the same range of zeatin concentration, the four genotypes gave higher multiplication rates on WPM medium. Acrossall experiments, the highest multiplication rate (4.8) was obtained for ´Berkeley´ on WPM medium with the highest concentration 2 mg/l of zeatin. After three months of storage under normal growing conditions, the survival rate was very h

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/QH82232" target="_blank" >QH82232: Výzkum opomíjených ovocných druhů a jejich začlenění do agrárního sektoru.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agriculture

ISSN

0551-3677

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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