Occurrence of potential vectors of phytoplasma in pear orchards with different plantation management

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F14%3A%230000543" target="_blank" >RIV/25271121:_____/14:#0000543 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.agriculturejournals.cz/publicFiles/130004.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/130004.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of potential vectors of phytoplasma in pear orchards with different plantation management

Popis výsledku v původním jazyce

During 2009?2011, regular captures of Cacopsylla pyri, C. pyrisuga and C. pyricola in four pear orchards (one conventional orchard, one integrated pest management (IPM) orchard, two organic orchards) were conducted in the Czech Republic. Polymerase chainreaction (PCR) tests were performed on psyllids captured from the orchards with the highest occurrence of these insects. The DNA extracted from pairs of psyllid individuals was analysed by nested PCR (R16F2n/R2-fU5/rU3) and restriction fragment length polymorphism method (RFLP) (RsaI, BfmI). Two C. pyri individuals captured on 11/05/2011 in the IPM orchard tested positive for Ca. P. pyri. Ca. P. pyri presence was confirmed in 8 out of the 9 Cacopsylla samples tested by subsequent nested PCR (P1/P7- f01/r01). In 2010 a higher amount of C. pyri was captured in the conventional orchard during June/July and in the IPM orchard during March, May, June and July. In 2010 and 2011 no or lower psyllids presence was detected in the organic orchar

Název v anglickém jazyce

Occurrence of potential vectors of phytoplasma in pear orchards with different plantation management

Popis výsledku anglicky

During 2009?2011, regular captures of Cacopsylla pyri, C. pyrisuga and C. pyricola in four pear orchards (one conventional orchard, one integrated pest management (IPM) orchard, two organic orchards) were conducted in the Czech Republic. Polymerase chainreaction (PCR) tests were performed on psyllids captured from the orchards with the highest occurrence of these insects. The DNA extracted from pairs of psyllid individuals was analysed by nested PCR (R16F2n/R2-fU5/rU3) and restriction fragment length polymorphism method (RFLP) (RsaI, BfmI). Two C. pyri individuals captured on 11/05/2011 in the IPM orchard tested positive for Ca. P. pyri. Ca. P. pyri presence was confirmed in 8 out of the 9 Cacopsylla samples tested by subsequent nested PCR (P1/P7- f01/r01). In 2010 a higher amount of C. pyri was captured in the conventional orchard during June/July and in the IPM orchard during March, May, June and July. In 2010 and 2011 no or lower psyllids presence was detected in the organic orchar

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QH92179" target="_blank" >QH92179: Zvýšení účinnosti integrované ochrany jádrovin proti komplexu škodlivých činitelů zaváděním biologických prostředků a podporou biodiverzity agroekosystémů sadů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Horticultural Science

ISSN

1805-9333

e-ISSN

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Svazek periodika

41

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

107-113

Kód UT WoS článku

000343363300002

EID výsledku v databázi Scopus

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