Micropropagation of Rosa pomifera

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F14%3A%230000582" target="_blank" >RIV/25271121:_____/14:#0000582 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Micropropagation of Rosa pomifera

Popis výsledku v původním jazyce

The purpose of this study was to develop an efficient micropropagation system for edible cultivars of apple rose (Rosa pomifera). Two genotypes, 'Karpatia' and 'Belecska', were successfully established in vitro using mercuric chloride or sodium hypochlorite as sterilization solutions. In total only 19.2% of the initial explants of both genotypes were found to be contaminated with micro-organisms, all from explants treated with sodium hypochlorite. Six MS-based media containing 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine), O.S or 1 mg L-1 TDZ (thidiazuron) or 10 mg. L-1 2iP (6-(y,y-dimethylallylamino) purine) were tested for initiation of multiplication. Generally, the highest proliferation rate (5.1) was obtained for 'Belecska' on MS medium with the highest concentration of BAP (4 mg L-1 ). The lowest shoot number (1.1) was noted for cultivar 'Karpatia' on MS medium with 1 mg L-1 TDZ. Abundant callus formation at the base of explants and abnormally narrow undeveloped leaves were observed

Název v anglickém jazyce

Micropropagation of Rosa pomifera

Popis výsledku anglicky

The purpose of this study was to develop an efficient micropropagation system for edible cultivars of apple rose (Rosa pomifera). Two genotypes, 'Karpatia' and 'Belecska', were successfully established in vitro using mercuric chloride or sodium hypochlorite as sterilization solutions. In total only 19.2% of the initial explants of both genotypes were found to be contaminated with micro-organisms, all from explants treated with sodium hypochlorite. Six MS-based media containing 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine), O.S or 1 mg L-1 TDZ (thidiazuron) or 10 mg. L-1 2iP (6-(y,y-dimethylallylamino) purine) were tested for initiation of multiplication. Generally, the highest proliferation rate (5.1) was obtained for 'Belecska' on MS medium with the highest concentration of BAP (4 mg L-1 ). The lowest shoot number (1.1) was noted for cultivar 'Karpatia' on MS medium with 1 mg L-1 TDZ. Abundant callus formation at the base of explants and abnormally narrow undeveloped leaves were observed

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

9789462610361

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

6

Strana od-do

215-220

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Nelson, New Zealand

Datum konání akce

25. 3. 2012

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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