Micropropagation of blackberry genotypes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F16%3AN0000007" target="_blank" >RIV/25271121:_____/16:N0000007 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1133.75" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1133.75</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1133.75" target="_blank" >10.17660/ActaHortic.2016.1133.75</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micropropagation of blackberry genotypes

Popis výsledku v původním jazyce

The purpose of this study was to develop an efficient in vitro multiplication system for blackberry (Rubus fruticosus L.). A successful in vitro culture technique would provide an alternative method, which can potentially multiply selected blackberry genotypes more rapidly than traditional nursery systems. Two genotypes, 'Cacanska bestrna' and blackberry selection No. 7, were sterilised using 0.15% mercuric chloride as sterilization solution. The use of mercuric chloride had a direct beneficial effect to overcome the contamination from all starting plant material. Four types of modified MS medium containing 1, 2 or 4 mg L-1 6-benzylaminopurine (BAP) or combination of 1 mg L-1 BAP, 0.1 mg L-1 indole-3-butyric acid (IBA) and 0.1 mg L-1 gibberellic acid GA (3) were tested in multiplication stage. Generally, the highest proliferation rate was obtained for cultivar Tatanska bestrna' that produced 6.3 shoots (longer than 10 mm) on a medium containing 4 mg L-1 BAP. All in vitro shoots started spontaneous rooting on multiplication media after depletion of cytokinines after 1 month ofcultivation. High survival (more than 95%) was obtained after acclimatization. The plants established after a period of in vitro culture showed no visible morphological differences compared with conventionally propagated plants. In conclusion, R. fruticosus can be efficiently propagated using a modified MS medium.

Název v anglickém jazyce

Micropropagation of blackberry genotypes

Popis výsledku anglicky

The purpose of this study was to develop an efficient in vitro multiplication system for blackberry (Rubus fruticosus L.). A successful in vitro culture technique would provide an alternative method, which can potentially multiply selected blackberry genotypes more rapidly than traditional nursery systems. Two genotypes, 'Cacanska bestrna' and blackberry selection No. 7, were sterilised using 0.15% mercuric chloride as sterilization solution. The use of mercuric chloride had a direct beneficial effect to overcome the contamination from all starting plant material. Four types of modified MS medium containing 1, 2 or 4 mg L-1 6-benzylaminopurine (BAP) or combination of 1 mg L-1 BAP, 0.1 mg L-1 indole-3-butyric acid (IBA) and 0.1 mg L-1 gibberellic acid GA (3) were tested in multiplication stage. Generally, the highest proliferation rate was obtained for cultivar Tatanska bestrna' that produced 6.3 shoots (longer than 10 mm) on a medium containing 4 mg L-1 BAP. All in vitro shoots started spontaneous rooting on multiplication media after depletion of cytokinines after 1 month ofcultivation. High survival (more than 95%) was obtained after acclimatization. The plants established after a period of in vitro culture showed no visible morphological differences compared with conventionally propagated plants. In conclusion, R. fruticosus can be efficiently propagated using a modified MS medium.

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

—

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of XI International Rubus nad Ribes Symposium

ISBN

978-946261115-3

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

4

Strana od-do

487-490

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven, Belgium

Místo konání akce

Asheville, NC

Datum konání akce

1. 1. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000391239400074