In vitro multiplication and rooting of two Czech sweet cherry cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000036" target="_blank" >RIV/25271121:_____/17:N0000036 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1161.49" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1161.49</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1161.49" target="_blank" >10.17660/ActaHortic.2017.1161.49</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro multiplication and rooting of two Czech sweet cherry cultivars

Popis výsledku v původním jazyce

The goal of this study was to identify factors affecting in vitro shoot and root production of two Czech sweet cherry (Prunus avium) cultivars, 'Amid' and 'Kares Frühe' under in vitro culture conditions. This information is required prior to experiments with chemotherapy as a method of sanitation from viruses. The two genotypes were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 16.7%. Six proliferation MS media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine), 0.5 and 1 mg L-1 TDZ (thidiazuron) or 10 mg L-1 2iP (6-(g,g-dimethylallylamino) purine) were tested for effects on proliferation, callus formation and shoot morphology. Multiplication rate values were relatively low and varied between 1.1 and 2.1. BAP at 4 mg L-1 was found to be more effective than TDZ and 2iP for shoot multiplication. Rooting was promoted on MS medium supplied with 1 mg L-1 NAA (naphthaleneacetic acid). Root initiation started within 2 weeks. The root induction was relatively low, 45% for 'Kares Frühe' and 28% for 'Amid'. In conclusion, multiplication and rooting rates were sufficient for in vitro culture establishment, short-term maintenance and in vitro chemotherapy. However, these relatively low rates are not satisfactory for in vitro production of plants on a commercial scale.

Název v anglickém jazyce

In vitro multiplication and rooting of two Czech sweet cherry cultivars

Popis výsledku anglicky

The goal of this study was to identify factors affecting in vitro shoot and root production of two Czech sweet cherry (Prunus avium) cultivars, 'Amid' and 'Kares Frühe' under in vitro culture conditions. This information is required prior to experiments with chemotherapy as a method of sanitation from viruses. The two genotypes were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 16.7%. Six proliferation MS media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine), 0.5 and 1 mg L-1 TDZ (thidiazuron) or 10 mg L-1 2iP (6-(g,g-dimethylallylamino) purine) were tested for effects on proliferation, callus formation and shoot morphology. Multiplication rate values were relatively low and varied between 1.1 and 2.1. BAP at 4 mg L-1 was found to be more effective than TDZ and 2iP for shoot multiplication. Rooting was promoted on MS medium supplied with 1 mg L-1 NAA (naphthaleneacetic acid). Root initiation started within 2 weeks. The root induction was relatively low, 45% for 'Kares Frühe' and 28% for 'Amid'. In conclusion, multiplication and rooting rates were sufficient for in vitro culture establishment, short-term maintenance and in vitro chemotherapy. However, these relatively low rates are not satisfactory for in vitro production of plants on a commercial scale.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210175" target="_blank" >QJ1210175: Výzkum a vývoj standardních metodických postupů ozdravování ovocných dřevin a révy vinné pomocí chemoterapie in vitro kultur pro systém certifikace zdravotního stavu výsadbového materiálu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-62611-57-3

ISSN

0567-7572

e-ISSN

2406-6168

Počet stran výsledku

5

Strana od-do

303-307

Název nakladatele

ISHS

Místo vydání

Leuven 1, Belgium

Místo konání akce

Plasencia, Spain

Datum konání akce

23. 6. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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