Use of X-ray-induced mutation in sweet cherry breeding

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000086" target="_blank" >RIV/25271121:_____/23:N0000086 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.actahort.org/books/1362/1362_26.htm" target="_blank" >https://www.actahort.org/books/1362/1362_26.htm</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of X-ray-induced mutation in sweet cherry breeding

Popis výsledku v původním jazyce

An article of Acta Horticulturae symposium proceedings (ISHS Acta Horticulturae 1362: XXXI International Horticultural Congress (IHC2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops) Sweet cherry (Prunus avium L.) breeding programs produce commercial cuiltivars primarily using traditional breeding methods. Induced mutations and molecular biology methods represent a tool for acceleration and refining the breeding process. An increasingly desired feature is the self-pollination of sweet cherries. They are primarily self-incompatible, but some gene mutations can enable the self-compatibility. We induced the mutation of genes leading to self-pollination at the RBIP Holovousy in the Czech Republic. Branches with generative buds in the meiotic phase of pollen grains of sweet cherry cultivars ‘Tamara’, ‘Irena’ and ‘Burlat’ were irradiated with X-rays at a dose of 20 Gy. In 3 years, 493 sweet cherry embryos were sown, out of which 34 individuals were raised. They were then subjected to fragment analyses to determine the combination of S-alleles. An individual with a combination of S-alleles: mutated S4’S6 was identified in the genotypes. The initial combination of S-alleles was S4S6. In this individual, a mutation in the S4 allele is expected and will be verified by genotyping using SSR markers.

Název v anglickém jazyce

Use of X-ray-induced mutation in sweet cherry breeding

Popis výsledku anglicky

An article of Acta Horticulturae symposium proceedings (ISHS Acta Horticulturae 1362: XXXI International Horticultural Congress (IHC2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops) Sweet cherry (Prunus avium L.) breeding programs produce commercial cuiltivars primarily using traditional breeding methods. Induced mutations and molecular biology methods represent a tool for acceleration and refining the breeding process. An increasingly desired feature is the self-pollination of sweet cherries. They are primarily self-incompatible, but some gene mutations can enable the self-compatibility. We induced the mutation of genes leading to self-pollination at the RBIP Holovousy in the Czech Republic. Branches with generative buds in the meiotic phase of pollen grains of sweet cherry cultivars ‘Tamara’, ‘Irena’ and ‘Burlat’ were irradiated with X-rays at a dose of 20 Gy. In 3 years, 493 sweet cherry embryos were sown, out of which 34 individuals were raised. They were then subjected to fragment analyses to determine the combination of S-alleles. An individual with a combination of S-alleles: mutated S4’S6 was identified in the genotypes. The initial combination of S-alleles was S4S6. In this individual, a mutation in the S4 allele is expected and will be verified by genotyping using SSR markers.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910290" target="_blank" >QK1910290: Rozvoj a aplikace molekulárně genetických metod pro racionalizaci šlechtitelských postupů třešní (Prunus avium L.)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

ISHS Acta Horticulturae 1362 XXXI International Horticultural Congress (IHC2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops

ISBN

978-94-62613-61-4

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

195-198

Název nakladatele

ISHS

Místo vydání

Leuven, Belgium

Místo konání akce

Angers, Francie

Datum konání akce

14. 8. 2022

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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