Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR – Comparison of the Two Methods and with Visual Stand Evaluation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F17%3AN0000014" target="_blank" >RIV/25328859:_____/17:N0000014 - isvavai.cz</a>

Výsledek na webu

<a href="https://acta.mendelu.cz/media/pdf/actaun_2017065010205.pdf" target="_blank" >https://acta.mendelu.cz/media/pdf/actaun_2017065010205.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201765010205" target="_blank" >10.11118/actaun201765010205</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR – Comparison of the Two Methods and with Visual Stand Evaluation

Popis výsledku v původním jazyce

During 2013–2015, oilseed rape samples from various locations in the Czech Republic were analysed for the presence of Verticillium wilt. Samples were evaluated in the lab using ELISA and PCR as well as by visual evaluation during the samples collection. A comparison of detection match in individual methods also was made. ELISA and PCR matched in detecting Verticillium wilt in 60 % of cases. For practical use, a higher match rate would be necessary in cases where samples were to be analysed in various laboratories using only one of these techniques. The possibility was demonstrated to use kits as well as primers not targeted specifically to just Verticillium longisporum but recording both main species on oilseed rape (V. longisporum and V. dahliae). The match rates of lab analyses with visual evaluation of stand infection were surprisingly high at 62 % for ELISA and 77 % for PCR. All three stand evaluation methods matched in 56 % of cases.

Název v anglickém jazyce

Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR – Comparison of the Two Methods and with Visual Stand Evaluation

Popis výsledku anglicky

During 2013–2015, oilseed rape samples from various locations in the Czech Republic were analysed for the presence of Verticillium wilt. Samples were evaluated in the lab using ELISA and PCR as well as by visual evaluation during the samples collection. A comparison of detection match in individual methods also was made. ELISA and PCR matched in detecting Verticillium wilt in 60 % of cases. For practical use, a higher match rate would be necessary in cases where samples were to be analysed in various laboratories using only one of these techniques. The possibility was demonstrated to use kits as well as primers not targeted specifically to just Verticillium longisporum but recording both main species on oilseed rape (V. longisporum and V. dahliae). The match rates of lab analyses with visual evaluation of stand infection were surprisingly high at 62 % for ELISA and 77 % for PCR. All three stand evaluation methods matched in 56 % of cases.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1310227" target="_blank" >QJ1310227: Nové poznatky z biologie a epidemiologie patogenů řepky a jejich rezistence k pesticidům v podmínkách České republiky jako základy racionalizace ochrany proti nim</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACTA UNIVERSITATIS AGRICULTURAE ET SILVICULTURAE MENDELIANAE BRUNENSIS

ISSN

1211-8516

e-ISSN

2464-8310

Svazek periodika

65

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

205-210

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85015869734