Mutations in target genes of succinate dehydrogenase inhibitors and demethylation inhibitors in Ramularia collo-cygni in Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F19%3AN0000010" target="_blank" >RIV/25328859:_____/19:N0000010 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/19:73596553

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s41348-019-00246-4" target="_blank" >https://link.springer.com/article/10.1007/s41348-019-00246-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s41348-019-00246-4" target="_blank" >10.1007/s41348-019-00246-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mutations in target genes of succinate dehydrogenase inhibitors and demethylation inhibitors in Ramularia collo-cygni in Europe

Popis výsledku v původním jazyce

Ramularia collo-cygni is a devastating plant pathogen of barley with an increasing importance in Europe and several other barley growing regions worldwide. R. collo-cygni is a pathogen with potential to adapt rapidly to environmental changes and is classified as a "high-risk" phytopathogen with respect to the evolution of fungicide resistance. Quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs) represent three major fungicide classes that are frequently used to control a broad range of relevant cereal pathogens. However, in recent years R. collo-cygni has acquired resistance to QoIs; in addition reduced efficacy of SDHI- and DMI-containing products has been observed. In this study, we have investigated the frequency of SDHI- and DMI-adapted isolates in a European population of R. collo-cygni and evaluated the underlying resistance mechanisms towards both fungicide classes. Several mutations in the target genes of SDHIs were detected in the population of R. collo-cygni under investigation (B-H266Y/R, B-T267I, B-I268V, C-N87S, C-H146R, C-H153R and some others) with increasing frequencies since 2014. SDHI resistance in R. collo-cygni is mainly driven by both the presence of mutations and the high frequency of such mutations in the population. Additionally, DMI-adapted isolates of R. collo-cygni were found at a high frequency in the countries under investigation. Fifteen different Cyp51 haplotypes were detected in the set of isolates from 2009 to 2017. The most frequent haplotype in 2017 was C1 haplotype, which comprises three mutations in Cyp51 and shows a substantial increase in EC50 values to DMIs compared with other isolates. As R. collo-cygni has adapted to several groups of fungicides in many regions in Western Europe, future control of this highly diverse and adaptable pathogen must focus on new molecules, choosing resistant varieties and the improvement in seed hygiene standards.

Název v anglickém jazyce

Mutations in target genes of succinate dehydrogenase inhibitors and demethylation inhibitors in Ramularia collo-cygni in Europe

Popis výsledku anglicky

Ramularia collo-cygni is a devastating plant pathogen of barley with an increasing importance in Europe and several other barley growing regions worldwide. R. collo-cygni is a pathogen with potential to adapt rapidly to environmental changes and is classified as a "high-risk" phytopathogen with respect to the evolution of fungicide resistance. Quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs) represent three major fungicide classes that are frequently used to control a broad range of relevant cereal pathogens. However, in recent years R. collo-cygni has acquired resistance to QoIs; in addition reduced efficacy of SDHI- and DMI-containing products has been observed. In this study, we have investigated the frequency of SDHI- and DMI-adapted isolates in a European population of R. collo-cygni and evaluated the underlying resistance mechanisms towards both fungicide classes. Several mutations in the target genes of SDHIs were detected in the population of R. collo-cygni under investigation (B-H266Y/R, B-T267I, B-I268V, C-N87S, C-H146R, C-H153R and some others) with increasing frequencies since 2014. SDHI resistance in R. collo-cygni is mainly driven by both the presence of mutations and the high frequency of such mutations in the population. Additionally, DMI-adapted isolates of R. collo-cygni were found at a high frequency in the countries under investigation. Fifteen different Cyp51 haplotypes were detected in the set of isolates from 2009 to 2017. The most frequent haplotype in 2017 was C1 haplotype, which comprises three mutations in Cyp51 and shows a substantial increase in EC50 values to DMIs compared with other isolates. As R. collo-cygni has adapted to several groups of fungicides in many regions in Western Europe, future control of this highly diverse and adaptable pathogen must focus on new molecules, choosing resistant varieties and the improvement in seed hygiene standards.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF PLANT DISEASES AND PROTECTION

ISSN

1861-3829

e-ISSN

1861-3837

Svazek periodika

126

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

447-459

Kód UT WoS článku

000491548600010

EID výsledku v databázi Scopus

2-s2.0-85068929551