Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F20%3AN0000003" target="_blank" >RIV/25328859:_____/20:N0000003 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61989592:15310/20:73603310 RIV/00027006:_____/20:10149297
Výsledek na webu
<a href="https://www.agriculturejournals.cz/publicFiles/52_2019-CJGPB.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/52_2019-CJGPB.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/52/2019-CJGPB" target="_blank" >10.17221/52/2019-CJGPB</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR
Popis výsledku v původním jazyce
The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017-2018. The assessment was carried out visually by symptoms and by a quantitative real- time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.
Název v anglickém jazyce
Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR
Popis výsledku anglicky
The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017-2018. The assessment was carried out visually by symptoms and by a quantitative real- time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Czech Journal of Genetics and Plant Breeding
ISSN
1212-1975
e-ISSN
1805-9325
Svazek periodika
56
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
9-14
Kód UT WoS článku
000501873500002
EID výsledku v databázi Scopus
2-s2.0-85075982071