Effect of melanin on activities of cytochrome P450 1A1 and 1A2

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F24%3AN0000041" target="_blank" >RIV/25328859:_____/24:N0000041 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of melanin on activities of cytochrome P450 1A1 and 1A2

Popis výsledku v původním jazyce

Lecture given at the First Joint Conference of Pharmacological Societies 2024, Ostrava 13 - 15 June 2024. The isolation and characterization of melanin from plant matrices using a modified version of the alkaline extraction method is described. Melanin was isolated from barley (Nudimelanocriton variety) and purified by a series of organic solvent treatments, acid hydrolysis and repeated precipitation. Approximately 5 mg of pure melanin was obtained from 12 g of barley grains. The isolated melanin showed its characteristic properties of insolubility in water, acids and organic solvents, while being soluble in alkaline media and precipitating below pH 3. The effect of melanin on cytochrome P450 1A1/2 enzyme activity in HepG2 cells was evaluated by 7-ethoxyresorufin O-dealkylation and high-performance liquid chromatography. Melanin at three concentrations (10 µg/ml, 1 µg/ml and 0.1 µg/ml) did not significantly induce cytochrome P450 1A1/2 enzyme activity (unlike the strong CYP1A1/2 inducer, 2,3,7,8-tetrachlorodibenzodioxin, which results in an almost sixty-fold increase). These findings contribute to the understanding of the physicochemical properties of melanin derived from barley and its interaction with liver enzymes of xenobiotic biotransformation (such as CYP1A1/2).

Název v anglickém jazyce

Effect of melanin on activities of cytochrome P450 1A1 and 1A2

Popis výsledku anglicky

Lecture given at the First Joint Conference of Pharmacological Societies 2024, Ostrava 13 - 15 June 2024. The isolation and characterization of melanin from plant matrices using a modified version of the alkaline extraction method is described. Melanin was isolated from barley (Nudimelanocriton variety) and purified by a series of organic solvent treatments, acid hydrolysis and repeated precipitation. Approximately 5 mg of pure melanin was obtained from 12 g of barley grains. The isolated melanin showed its characteristic properties of insolubility in water, acids and organic solvents, while being soluble in alkaline media and precipitating below pH 3. The effect of melanin on cytochrome P450 1A1/2 enzyme activity in HepG2 cells was evaluated by 7-ethoxyresorufin O-dealkylation and high-performance liquid chromatography. Melanin at three concentrations (10 µg/ml, 1 µg/ml and 0.1 µg/ml) did not significantly induce cytochrome P450 1A1/2 enzyme activity (unlike the strong CYP1A1/2 inducer, 2,3,7,8-tetrachlorodibenzodioxin, which results in an almost sixty-fold increase). These findings contribute to the understanding of the physicochemical properties of melanin derived from barley and its interaction with liver enzymes of xenobiotic biotransformation (such as CYP1A1/2).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QL24010230" target="_blank" >QL24010230: Barevná pšenice a ječmen – výzva pro budoucnost</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů