THE POSSIBILITIES FOR IDENTIFICATION AND CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CULTIVARS USING MICROSATELLITE MARKERS

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

THE POSSIBILITIES FOR IDENTIFICATION AND CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CULTIVARS USING MICROSATELLITE MARKERS

Popis výsledku v původním jazyce

THE AIM OF THIS STUDY WAS TO ESTABLISH POSSIBILITIES OF CHOSEN SSR MARKERS FOR THE IDENTIFICATION AND DIFFERENTIATION A SET OF 37 ALFALFA CULTIVARS (MEDICAGO SATIVA L.) AND ALSO THE UTILIZATION THESE SSR MARKERS TO DETECT GENETIC DIVERSITY AMONG ALFALFA CULTIVARS. A TOTAL OF 5 SSR MARKERS WERE SELECTED FROM PREVIOUS STUDIES BASED ON THEIR HIGH POLYMORPHISM IDENTIFICATION CONTENT (PIC). ALL USED MARKERS WERE POLYMORPHIC AND PRODUCED REPRODUCIBLE, CONSISTENT AND EASILY SCORABLE DATA. THESE FIVE PRIMER PAIRS AMPLIFIED ALTOGETHER 18 DIFFERENT POLYMORPHIC ALLELES WITH AN AVERAGE NUMBER OF 3.6 ALLELES PER LOCUS. THE NUMBER OF ALLELES RANGED FROM 3 (FMT13) TO 6 (AFCTT1). THE EXPECTED HETEROZYGOSITY (HE) AND POLYMORPHIC INFORMATION CONTENT (PIC) OF THE TESTED SSR MARKERS WERE CALCULATED. THE HE OF THE TESTED SSR MARKERS RANGED FROM 0.5737 (FMT13) TO 0.7267 (AFCTT1) WITH AN AVERAGE OF 0.6297. THE PIC RANGED FROM 0.4921 (MTIC430) TO 0.6788 (AFCTT1) WITH AN AVERAGE OF 0.5593. THE DENDROGRAM BASED ON HIERARCHICAL CLUSTER ANALYSIS USING UPGMA ALGORITHM FOR THE DETECTION OF GENETIC DIVERSITY WAS CONSTRUCTED. THE CULTIVARS WERE GROUPED INTO THREE MAIN CLUSTERS. IT WAS NOT POSSIBLE TO DISTINGUISH CULTIVARS KOLY (YEM) & REMANY (YEM) AND KM - MARARTON (HUN) & CRENO (DNK) FROM EACH OTHER, RESPECTIVELY.

Název v anglickém jazyce

THE POSSIBILITIES FOR IDENTIFICATION AND CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CULTIVARS USING MICROSATELLITE MARKERS

Popis výsledku anglicky

THE AIM OF THIS STUDY WAS TO ESTABLISH POSSIBILITIES OF CHOSEN SSR MARKERS FOR THE IDENTIFICATION AND DIFFERENTIATION A SET OF 37 ALFALFA CULTIVARS (MEDICAGO SATIVA L.) AND ALSO THE UTILIZATION THESE SSR MARKERS TO DETECT GENETIC DIVERSITY AMONG ALFALFA CULTIVARS. A TOTAL OF 5 SSR MARKERS WERE SELECTED FROM PREVIOUS STUDIES BASED ON THEIR HIGH POLYMORPHISM IDENTIFICATION CONTENT (PIC). ALL USED MARKERS WERE POLYMORPHIC AND PRODUCED REPRODUCIBLE, CONSISTENT AND EASILY SCORABLE DATA. THESE FIVE PRIMER PAIRS AMPLIFIED ALTOGETHER 18 DIFFERENT POLYMORPHIC ALLELES WITH AN AVERAGE NUMBER OF 3.6 ALLELES PER LOCUS. THE NUMBER OF ALLELES RANGED FROM 3 (FMT13) TO 6 (AFCTT1). THE EXPECTED HETEROZYGOSITY (HE) AND POLYMORPHIC INFORMATION CONTENT (PIC) OF THE TESTED SSR MARKERS WERE CALCULATED. THE HE OF THE TESTED SSR MARKERS RANGED FROM 0.5737 (FMT13) TO 0.7267 (AFCTT1) WITH AN AVERAGE OF 0.6297. THE PIC RANGED FROM 0.4921 (MTIC430) TO 0.6788 (AFCTT1) WITH AN AVERAGE OF 0.5593. THE DENDROGRAM BASED ON HIERARCHICAL CLUSTER ANALYSIS USING UPGMA ALGORITHM FOR THE DETECTION OF GENETIC DIVERSITY WAS CONSTRUCTED. THE CULTIVARS WERE GROUPED INTO THREE MAIN CLUSTERS. IT WAS NOT POSSIBLE TO DISTINGUISH CULTIVARS KOLY (YEM) & REMANY (YEM) AND KM - MARARTON (HUN) & CRENO (DNK) FROM EACH OTHER, RESPECTIVELY.

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

SEED AND SEEDLINGS

ISBN

978-80-213-2544-9

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

120-125

Název nakladatele

CZU Praha

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

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Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

000357378800034