The loss of polyphenol oxidase function is associated with hilum pigmentation and has been selected during pea domestication

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26296080%3A_____%2F22%3AN0000135" target="_blank" >RIV/26296080:_____/22:N0000135 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/22:73613916

Výsledek na webu

<a href="https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.18256" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.18256</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.18256" target="_blank" >10.1111/nph.18256</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The loss of polyphenol oxidase function is associated with hilum pigmentation and has been selected during pea domestication

Popis výsledku v původním jazyce

Seed coats serve as protective tissue to the enclosed embryo. As well as mechanical there are also chemical defence functions. During domestication, the property of the seed coat was altered including the removal of the seed dormancy. We used a range of genetic, transcriptomic, proteomic and metabolomic approaches to determine the function of the pea seed polyphenol oxidase (PPO) gene. Sequencing analysis revealed one nucleotide insertion or deletion in the PPO gene, with the functional PPO allele found in all wild pea samples, while most cultivated peas have one of the three nonfunctional ppo alleles. PPO functionality cosegregates with hilum pigmentation. PPO gene and protein expression, as well as enzymatic activity, was downregulated in the seed coats of cultivated peas. The functionality of the PPO gene relates to the oxidation and polymerisation of gallocatechin in the seed coat. Additionally, imaging mass spectrometry supports the hypothesis that hilum pigmentation is conditioned by the presence of both phenolic precursors and sufficient PPO activity. Taken together these results indicate that the nonfunctional polyphenol oxidase gene has been selected during pea domestication, possibly due to better seed palatability or seed coat visual appearance.

Název v anglickém jazyce

The loss of polyphenol oxidase function is associated with hilum pigmentation and has been selected during pea domestication

Popis výsledku anglicky

Seed coats serve as protective tissue to the enclosed embryo. As well as mechanical there are also chemical defence functions. During domestication, the property of the seed coat was altered including the removal of the seed dormancy. We used a range of genetic, transcriptomic, proteomic and metabolomic approaches to determine the function of the pea seed polyphenol oxidase (PPO) gene. Sequencing analysis revealed one nucleotide insertion or deletion in the PPO gene, with the functional PPO allele found in all wild pea samples, while most cultivated peas have one of the three nonfunctional ppo alleles. PPO functionality cosegregates with hilum pigmentation. PPO gene and protein expression, as well as enzymatic activity, was downregulated in the seed coats of cultivated peas. The functionality of the PPO gene relates to the oxidation and polymerisation of gallocatechin in the seed coat. Additionally, imaging mass spectrometry supports the hypothesis that hilum pigmentation is conditioned by the presence of both phenolic precursors and sufficient PPO activity. Taken together these results indicate that the nonfunctional polyphenol oxidase gene has been selected during pea domestication, possibly due to better seed palatability or seed coat visual appearance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA19-07155S" target="_blank" >GA19-07155S: Identifikace regulačních sítí kontrolujících vývoj osemení hrachu pomocí RNA sekvenování, proteinové a metabolomické analýzy.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

1469-8137

Svazek periodika

235

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

1807-1821

Kód UT WoS článku

000809206100001

EID výsledku v databázi Scopus

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