Fungi and bacterial life of healthy and diseased red clover (Trifolium pratense L.) roots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26296080%3A_____%2F25%3AN0000051" target="_blank" >RIV/26296080:_____/25:N0000051 - isvavai.cz</a>

Výsledek na webu

<a href="https://6thplantmicrobiomesymposium2025.com/wp-content/uploads/2025/11/PMS2025_AbstractBook.pdf" target="_blank" >https://6thplantmicrobiomesymposium2025.com/wp-content/uploads/2025/11/PMS2025_AbstractBook.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fungi and bacterial life of healthy and diseased red clover (Trifolium pratense L.) roots

Popis výsledku v původním jazyce

Root diseases are a significant issue in red clover (Trifolium pratense L.), reducing its forage yield, diminishing nutritional quality, and increasing mycotoxin risk. Root rot in red clover is mainly caused by Fusarium spp., although other fungal pathogens may also contribute. This study investigates the fungal and bacterial communities associated with healthy and diseased red clover roots, using ITS and 16S rDNA sequencing. In total, 146 root samples were collected from 15 locations across the Moravian region of the Czech Republic. The objective was to identify bacterial and fungal taxa associated with disease status and to understand their potential roles in plant health and disease progression. The most abundant fungal genera include Cladosporium, Plectosphaerella, Didymella, Gibellulopsis, and Fusarium. As expected, several Fusarium species complexes (FIESC, FOSC, FSAMSC, FTSC, FRSC, FNSC, FSSC) were among the primary fungal pathogens associated with disease. Other common and statistically significant fungi associated with disease include Didymella, Plectosphaerella, Paraphoma, Vishniacozyma, Fusicolla, Clonostachys, Torula, and Neosetophoma. Among bacteria, Rhizobium that reflecting the symbiotic nitrogen fixation activity, was the most abundant genus of rhizosphere bacteria. Other frequent genera included Nakamurella, Mycobacterium, Pseudonocardia, Actinoplanes, and Nocardioides. The bacteria identified in infected roots mainly suggest active processes of organic matter degradation (e.g., Cellulomonas, Cellulosilyticum, Nocardioides) and nitrogen cycling (Phyllobacterium, Variibacter). Some non-pathogenic genera (Stenotrophomonas, Serratia, Achromobacter) may represent opportunistic colonizers that indicate a stress response. Some representatives of the genus Xanthomonas are the only potential pathogens among the bacteria associated with infected plants and may act as pathogens under certain conditions. Our results describe the dynamics of microbial colonization and interactions within the microbiome of both healthy and diseased red clover roots, emphasizing the potential role of these microbial communities in disease progression and providing a source of data for the development of plant health management strategies.

Název v anglickém jazyce

Fungi and bacterial life of healthy and diseased red clover (Trifolium pratense L.) roots

Popis výsledku anglicky

Root diseases are a significant issue in red clover (Trifolium pratense L.), reducing its forage yield, diminishing nutritional quality, and increasing mycotoxin risk. Root rot in red clover is mainly caused by Fusarium spp., although other fungal pathogens may also contribute. This study investigates the fungal and bacterial communities associated with healthy and diseased red clover roots, using ITS and 16S rDNA sequencing. In total, 146 root samples were collected from 15 locations across the Moravian region of the Czech Republic. The objective was to identify bacterial and fungal taxa associated with disease status and to understand their potential roles in plant health and disease progression. The most abundant fungal genera include Cladosporium, Plectosphaerella, Didymella, Gibellulopsis, and Fusarium. As expected, several Fusarium species complexes (FIESC, FOSC, FSAMSC, FTSC, FRSC, FNSC, FSSC) were among the primary fungal pathogens associated with disease. Other common and statistically significant fungi associated with disease include Didymella, Plectosphaerella, Paraphoma, Vishniacozyma, Fusicolla, Clonostachys, Torula, and Neosetophoma. Among bacteria, Rhizobium that reflecting the symbiotic nitrogen fixation activity, was the most abundant genus of rhizosphere bacteria. Other frequent genera included Nakamurella, Mycobacterium, Pseudonocardia, Actinoplanes, and Nocardioides. The bacteria identified in infected roots mainly suggest active processes of organic matter degradation (e.g., Cellulomonas, Cellulosilyticum, Nocardioides) and nitrogen cycling (Phyllobacterium, Variibacter). Some non-pathogenic genera (Stenotrophomonas, Serratia, Achromobacter) may represent opportunistic colonizers that indicate a stress response. Some representatives of the genus Xanthomonas are the only potential pathogens among the bacteria associated with infected plants and may act as pathogens under certain conditions. Our results describe the dynamics of microbial colonization and interactions within the microbiome of both healthy and diseased red clover roots, emphasizing the potential role of these microbial communities in disease progression and providing a source of data for the development of plant health management strategies.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TQ03000327" target="_blank" >TQ03000327: Detekce významných houbových patogenů kořene jetele lučního a jejich korelace s obsahem mykotoxinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů