Determination of selenium nanoparticles in fermented dairy products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F23%3AN0000001" target="_blank" >RIV/26722861:_____/23:N0000001 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/23:43925924

Výsledek na webu

<a href="https://doi.org/10.1016/j.sab.2022.106592" target="_blank" >https://doi.org/10.1016/j.sab.2022.106592</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sab.2022.106592" target="_blank" >10.1016/j.sab.2022.106592</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of selenium nanoparticles in fermented dairy products

Popis výsledku v původním jazyce

A single particle inductively coupled plasma mass spectrometry (sp-ICP-MS) method with improved sensitivity for determination of selenium nanoparticles (SeNPs) in lactic acid bacteria, bifidobacteria, and yeast was developed. The carbon-induced signal enhancement using 1% (v/v) methanol for preparation of sample dispersion led to decreasing detection limits from 61 nm to 47 nm in comparison to pure aqueous dispersion. Accuracy of SeNPs’ size and number concentration determination were tested by adding artificially prepared SeNPs to the cells dispersion. While the found size of the SeNPs corresponded to original value, the number concentration recovery ranged from 46 to 102%. The repeatability of SeNPs size measurement was 0.6–15% and of number concentration determination 2.5–54%. The spherical and ovoid shaped SeNPs with average diameter in range of 60–261 nm and number concentration of 2.4 × 109–1.6 × 1011 mL-1 located both extracellularly and intracellularly was found by transmission electron microscopy and sp-ICP-MS in cell cultures growing in medium containing 10 mg L-1 of sodium selenite. Bio-synthesized SeNPs were stable for one week in aqueous dispersion and their stability was prolonged to one month by addition of hydrochloric acid (pH = 1.5). Moreover, the possibility of analyzing SeNPs in enzymatic extracts intended for speciation analysis of organic selenium species was proven to be suitable also for SeNPs characterization. Thus, a way to share sample preparation procedure with different types of analyses was demonstrated.

Název v anglickém jazyce

Determination of selenium nanoparticles in fermented dairy products

Popis výsledku anglicky

A single particle inductively coupled plasma mass spectrometry (sp-ICP-MS) method with improved sensitivity for determination of selenium nanoparticles (SeNPs) in lactic acid bacteria, bifidobacteria, and yeast was developed. The carbon-induced signal enhancement using 1% (v/v) methanol for preparation of sample dispersion led to decreasing detection limits from 61 nm to 47 nm in comparison to pure aqueous dispersion. Accuracy of SeNPs’ size and number concentration determination were tested by adding artificially prepared SeNPs to the cells dispersion. While the found size of the SeNPs corresponded to original value, the number concentration recovery ranged from 46 to 102%. The repeatability of SeNPs size measurement was 0.6–15% and of number concentration determination 2.5–54%. The spherical and ovoid shaped SeNPs with average diameter in range of 60–261 nm and number concentration of 2.4 × 109–1.6 × 1011 mL-1 located both extracellularly and intracellularly was found by transmission electron microscopy and sp-ICP-MS in cell cultures growing in medium containing 10 mg L-1 of sodium selenite. Bio-synthesized SeNPs were stable for one week in aqueous dispersion and their stability was prolonged to one month by addition of hydrochloric acid (pH = 1.5). Moreover, the possibility of analyzing SeNPs in enzymatic extracts intended for speciation analysis of organic selenium species was proven to be suitable also for SeNPs characterization. Thus, a way to share sample preparation procedure with different types of analyses was demonstrated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/LTC20014" target="_blank" >LTC20014: Research of selenium-enriched lactic acid bacteria, bifidobacteria and yeasts and influence of low-pH stress exposure on their selenium biotransformation efficiency.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Spectrochimica Acta - Part B Atomic Spectroscopy

ISSN

0584-8547

e-ISSN

1873-3565

Svazek periodika

199

Číslo periodika v rámci svazku

January 2023

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

106592

Kód UT WoS článku

000922665300011

EID výsledku v databázi Scopus

2-s2.0-85143352453