Studium genetické a epigenetické stability dlouhodobé in vitro kultury hrachu (Pisum sativum L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F07%3A%230000045" target="_blank" >RIV/26784246:_____/07:#0000045 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)

Popis výsledku v původním jazyce

We have used several molecular methods to assess the genetic stability of long-term propagated (24 years) multiple shoot in vitro culture of pea (Pisum sativum L.). We focused on assessing the stability of repetitive sequences, such as simple sequence repeats (SSR) and retrotransposons, both comprising a large part of genome. No differences were found between controls and long-term or newly established in vitro (one subculture cycle) samples were investigated by the SSR, inter-repeats (ISSR) or inter-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and the methylation sensitive MSAP methods detected 11 and 18% polymorphism. Some evidence of retrotransposon re-arrangement was observed by sequence-specific amplification polymorphism. Based on these results, multiple shoot culture of pea maintained over a long period may be considered as a true to type multiplication method of the original genotype.

Název v anglickém jazyce

Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)

Popis výsledku anglicky

We have used several molecular methods to assess the genetic stability of long-term propagated (24 years) multiple shoot in vitro culture of pea (Pisum sativum L.). We focused on assessing the stability of repetitive sequences, such as simple sequence repeats (SSR) and retrotransposons, both comprising a large part of genome. No differences were found between controls and long-term or newly established in vitro (one subculture cycle) samples were investigated by the SSR, inter-repeats (ISSR) or inter-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and the methylation sensitive MSAP methods detected 11 and 18% polymorphism. Some evidence of retrotransposon re-arrangement was observed by sequence-specific amplification polymorphism. Based on these results, multiple shoot culture of pea maintained over a long period may be considered as a true to type multiplication method of the original genotype.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Reports

ISSN

0721-7714

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

1985-1998

Kód UT WoS článku

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EID výsledku v databázi Scopus

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